Functional analysis and regulation of the divergent spuABCDEFGH-spuI operons for polyamine uptake and utilization in Pseudomonas aeruginosa PAO1.
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Spermidine uptake is greatly reduced in spuD and spuE mutants.
"Second, spermidine uptake is inducible in spermidine-grown cells and is greatly reduced in either spuD or spuE mutant (Fig."
Structural basis of substrate binding specificity revealed by the crystal structures of polyamine receptors SpuD and SpuE from Pseudomonas aeruginosa.
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SpuE binds spermidine exclusively.
"we found that SpuD is a putrescine-preferential binding protein, while SpuE binds to spermidine exclusively."
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Trp271 determines SpuE's spermidine specificity.
"substitution of Trp271 by Phe enabled SpuE to gain substantial binding affinity for putrescine"
UniProt entry Q9I6J0
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SpuE binds spermidine with high affinity and does not bind putrescine.
"Binds spermidine with high affinity (KD=14.3 nM). Does not bind putrescine, cadaverine or spermine."
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods