MITF (donkey, A0A9L0JBN5) - review notes

DONKEY40 cohort. 8 IEA GOA rows (TreeGrafter x4, InterPro2GO x2, subcellular-location mapping, combined).

Sequence

[file:EQUAS/MITF/MITF-bioinformatics/RESULTS.md "98.38% identity over 495 paired residues"]; bHLH ["54 of 54 human residues in this span are identical"]. Donkey residues 1-115 have no partner in human canonical O75030 (isoform/first-exon difference); human 138-162 and 293-298 absent in donkey model.

[file:EQUAS/MITF/MITF-bioinformatics/human-O75030-uniprot.txt "Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis"]
["Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR)"]
Localization regulated by mTORC1: ["Conversely, inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus"]; PMID:36608670

Accept DNA binding, RNAPII TF activity, nucleus, regulation of transcription by RNAPII, melanocyte differentiation. Cytoplasm and dimerization non-core. GO:0006355 too general.
ProtNLM2 GO:0006355: correct but less precise than GOA GO:0006357 (LSP).

ProtNLM2 output was not opened or used in this re-review.

just deep-research-falcon EQUAS MITF --fallback perplexity-lite failed: falcon "Failed to authenticate"; perplexity-lite HTTP 401 insufficient_quota. No deep-research file was produced. Literature was read directly from cached publications instead (PMID:9647758, PMID:23401004, PMID:16822840, PMID:36608670 and the human UniProt record O75030).

[file:EQUAS/MITF/MITF-bioinformatics/RESULTS-rereview.md "human isoform A1 residues 1-20 (MQSESGIVPDFEVGEEFHEE) found in donkey at 1-based position: 116"]. From residue 116 onwards the model is an isoform A-type product.
Residues 1-115 make up an Arg/Pro/Gly/Ser-rich extension ("R+P+G+S fraction: 0.55") with no counterpart among the 12 human isoforms in O75030. It is probably translated 5' UTR from an upstream in-frame start, i.e. a gene-model artefact, but that has not been shown.
It lacks the X1-type ACIFPT insert (so it is X2/A2-like) and a 25-residue segment matching human A1 138-162 (QVKQYLSTTLANKHANQVLSLPCPN).
Donkey has 5 TrEMBL MITF models (A0A8C4PGB1 641 aa, A0A9L0IUI7 504, A0A9L0JHX8 419 = M-length, A0A9L0KEI8 519, A0A9L0JBN5 610), all from Ensembl gene ENSEASG00005002172.2. None is reviewed, so there is no canonical Swiss-Prot product of the same gene to review. bHLH-Zip and C-terminal regions are intact (RESULTS.md, bHLH 54/54 identical).

UniProt places this model in PANTHER PTHR45776:SF4 (MICROPHTHALMIA-ASSOCIATED TRANSCRIPTION FACTOR), the same subfamily as human O75030, mouse Q08874 and rat O88368 (interpro/panther/PTHR45776/PTHR45776-entries.csv). The task header said no PANTHER id was listed, but the UniProt record does list one.
PAINT (interpro/panther/PTHR45776/PTHR45776-paint.tsv): GO:0000978, GO:0000981, GO:0006357 and GO:0005634 are IBDs at the MiT/TFE root PTN005248237. GO:0030318 melanocyte differentiation is an IBD on the MITF lineage only: PTN000000820 (Tetrapoda, seed MGI:104554 Mitf) and PTN000000863 (teleost mitfa/mitfb). That makes it subfamily-specific, and SF4 membership justifies transferring it. TreeGrafter transferred all four GOA TreeGrafter rows from PTN000000842.
There is no disagreement between this placement and the PANTHER family files. No family review YAML exists for PTHR45776 (only entries/metadata/paint files).

GO:0006357: ACCEPT -> MODIFY to GO:0045944 positive regulation of transcription by RNA polymerase II. The term is correct but general; MITF-A activates TYR/TYRP1 PMID:9647758.
GO:0006355: TOO_GENERAL -> MODIFY to GO:0045944, for the same reason.
core_functions: directly_involved_in now uses GO:0045944 in place of GO:0006357.
Unchanged: GO:0000978, GO:0000981, GO:0005634 and GO:0030318 ACCEPT; GO:0005737 and GO:0046983 KEEP_AS_NON_CORE. Support was strengthened with PAINT node rows and with Rag/14-3-3 localization papers PMID:23401004.
Lysosome localization (Rag recruitment) was not proposed as NEW, because it is a regulatory sequestration site and not a site of function.

Human MITF O75030 is an ortholog in the same PANTHER subfamily SF4 (PAINT seed for the root IBDs). There is no genes/human/MITF review, so it needs review. The only existing MITF-family reviews are zebrafish mitfa/mitfb (genes/DANRE/).
Modules: none found in modules/ that model MITF. It is mentioned only in prose in diadenosine_tetraphosphate_turnover.yaml and in kit_signaling deep research.