sec-16A.1 (ZK512.5; UniProt P34643) - manual literature review

No deep-research provider was available for this review, so this file records a
manual literature survey (PubMed search plus cached full texts in publications/).

Search

PubMed query (sec-16 OR SEC-16 OR Sec16) AND (elegans) (2026-10-28) returned three
records: PMID:21478858, PMID:23840591 and PMID:35870495. PMID:35870495 is a
Drosophila tracheal study that mentions C. elegans only in passing and does not
study the worm protein; it was not used.

Identity and family

• UniProt P34643 (SC161_CAEEL), 1232 aa, gene sec-16A.1, ORF ZK512.5; two isoforms
  (a, b; b lacks residues 1-8).
• Pfam PF12932 (Sec16 central conserved domain) and PF12931 (TPR_Sec16 / Sec23-binding
  C-terminal domain); InterPro IPR024340 Sec16_CCD and IPR024298 Sec16_Sec23-bd;
  PANTHER PTHR13402:SF6. UniProt: "Belongs to the SEC16 family."
• Long N-terminal and internal stretches are predicted disordered (MobiDB-lite),
  typical of Sec16 proteins.
• The protein was identified as the worm Sec16 homologue by Witte et al.
  PMID:21478858.

Experimental findings

Witte et al. 2011 (PMID:21478858, full text)

• Sec16 is framed as the ERES scaffold PMID:21478858.
• SEC-16 immunoprecipitation from embryo extracts plus mass spectrometry identified
  19 reproducible partners; RNAi showed PMID:21478858.
• Direct binding to TFG-1 [PMID:21478858 "We found that a GST-fusion to SEC-16, but not GST alone, was capable of binding to recombinant TFG-1"; "These data confirm a direct biochemical association between SEC-16 and TFG-1."]. The TFG-1 N-terminus mediates the interaction.
• Localization: SEC-16 marks ER exit sites in the germline/oocytes PMID:21478858.
• Mutual dependence PMID:21478858.
• SEC-16 depletion disrupts ERES and COPII puncta PMID:21478858.
• SEC-16 recruits COPII in proportion to its own level at ERES PMID:21478858.
• Secretory pathway phenotype of SEC-16 depletion PMID:21478858.
• SEC-16 and TFG-1 co-fractionate in large (>800 kD) complexes; TFG-1 hexamers promote
  assembly of SEC-16/COPII complexes.

Ackema et al. 2013 (PMID:23840591, full text)

• Studies the ArfGEF GBF-1; SEC-16 is used as an anti-SEC-16 antibody ERES marker.
  PMID:23840591
• gbf-1(RNAi) does not change SEC-16 localization PMID:23840591.
• The paper does not describe SEC-16 in an ERGIC; "ERGIC" does not appear in the full
  text. UniProt nevertheless cites it for "Endoplasmic reticulum-Golgi intermediate
  compartment" localization.

Gaps

• No biochemical reconstitution of worm SEC-16 with Sec23/Sec24, Sec13/Sec31 or SAR-1.
• Roles of the paralogous worm Sec16-family genes (other sec-16 loci) relative to
  sec-16A.1 are not established in the literature found.
• No studies in somatic secretory tissues (intestine, hypodermis, neurons) were found.