Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
C. elegans screen identifies autophagy genes specific to multicellular organisms.
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Identified epg-5 as a metazoan-specific autophagy gene required for formation of degradative autolysosomes.
"EI24 and mEPG5 are required for formation of degradative autolysosomes"
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epg-2, -3, -4, and -5 define discrete genetic steps of the autophagy pathway.
"Genetic analysis reveals that epg-2, -3, -4, and -5 define discrete genetic steps of the autophagy pathway"
Autophagy genes function sequentially to promote apoptotic cell corpse degradation in the engulfing cell.
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EPG-5 is recruited to the outer surface of internalized apoptotic Q cell corpses in phagocytes.
"the autophagy proteins LGG-1, ATG-18, and EPG-5 are recruited from the phagocyte to the outer surface of internalized Q cell corpses"
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EPG-5 functions in the phagocyte to promote apoptotic cell degradation via phagosome maturation.
"atg-18 and epg-5 function in the phagocyte to promote Q cell corpse clearance"
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epg-5 mutants show delayed RAB-7 recruitment to phagosomes.
"We found that the recruitment of RAB-7 onto the phagosome was delayed from 70 ± 15 min in WT (n = 11) to 145 ± 66 min in atg-18 (n = 18) and 102 ± 30 min in epg-5 (n = 12) mutants"
The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by interacting with the HOPS subunit VPS39.
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LGG-2 controls autophagosome maturation and facilitates tethering with lysosomes through VPS-39.
"LGG-2 controls the maturation of LGG-1-positive autophagosomes and facilitates the tethering with the lysosomes through a direct interaction with the VPS-39 HOPS complex subunit"
PI3P phosphatase activity is required for autophagosome maturation and autolysosome formation.
Deep research summary for epg-5