The review was assembled from the official annotation export, actual phylogeny and original literature/datasets. No previous review or notes existed in the qualified target namespace. Each of the 31 contexts received a manual decision; the four broad parents are too general beside the existing specific annotations. Generic GK2 binding is removed for lack of informative function while the positive associations are retained.
The source evidence is centralized in GK-external-evidence.md. All four natural product sequences and the original urine atom are preserved in GK-annotation-contexts.json. The two functional splice entries follow official named-form mappings; engineered reagents remain evidence, not new natural products.
The private preparation has not yet run a genuine native fetch or seed. Subsequent native preparation must preserve the original/current-context join, actual cache metadata, existing source bytes and all unrelated lane artifacts. The first target history event must be a genuine CREATE because the target history namespace is absent.
The preparation described above is now complete. Genuine fetch and seeding produced 31 distinct annotation keys, with the original urine restriction restored explicitly. All 25 normal sources are present: thirteen unchanged reused files and twelve missing-only acquisitions. Native metadata supplies eleven availability flags and the final punctuation of one title. The previously read fuller assay source does not change any scientific decision, core or functional variant. The two current excerpts each remain in one public placement. The first history event is CREATE; affected-check outcomes are recorded after execution.
Default validation, strict matching of all 31 GOA contexts, the computed-status audit, genuine CREATE history validation, private rendering and scoped whitespace passed. The computed status is COMPLETE, with zero annotation advisories. The two UNDECIDED nucleus contexts remain explicit. All 25 normal source files remain byte-exact after acquisition or reuse. No whole-repository or merge-group execution is claimed.
GK uses ATP to phosphorylate glycerol, producing sn-glycerol-3-phosphate. This catalytic entry supplies glycerol utilization and lipid synthesis. PMID:37021775(https://pubmed.ncbi.nlm.nih.gov/37021775/)
Exon-18 inclusion changes kinetic behavior and distribution in the reported comparison; the imaging ambiguity is recorded for curator follow-up. PMID:15845384(https://pubmed.ncbi.nlm.nih.gov/15845384/)
The predicted membrane helix falls inside the alternatively spliced exon-18 segment, supporting product-dependent membrane association. This sequence evidence does not establish membrane orientation. UniProt:P32189
Glycerol kinase deficiency elevates free glycerol. A glycerol-sensitive triglyceride assay can consequently report pseudohypertriglyceridemia; this is distinct from increased triglyceride synthesis. PMID:8651297(https://pubmed.ncbi.nlm.nih.gov/8651297/)
GK contact with NR4A1 accompanies altered receptor DNA binding and reporter activity, supporting a secondary receptor-binding function. PMID:21988832(https://pubmed.ncbi.nlm.nih.gov/21988832/)
MitoCoP supports mitochondrial membership for a GK protein group, without independently assigning every splice product or resolving membrane topology. PMID:34800366(https://pubmed.ncbi.nlm.nih.gov/34800366/)
The specificity criterion is biological scope: retain the established glycerol-catabolic, glycerol-3-phosphate biosynthetic and triglyceride-biosynthetic roles. Mark their broader parents too general when they introduce no additional supported reaction or pathway direction. Apply that criterion to IBA, IMP and IEA alike; phylogenetic evidence remains valid.