Gene Ontology annotation based on UniPathway vocabulary mapping
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
A new ubiquitin ligase involved in p57KIP2 proteolysis regulates osteoblast cell differentiation.
Interactome mapping suggests new mechanistic details underlying Alzheimer's disease.
A proteome-scale map of the human interactome network.
A High-Density Map for Navigating the Human Polycomb Complexome.
A reference map of the human binary protein interactome.
The FBXL family of F-box proteins: variations on a theme.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Multimodal cell maps as a foundation for structural and functional genomics.
Fbxl12 triggers G1 arrest by mediating degradation of calmodulin kinase I.
AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex
NEDD8:AcM-UBE2M binds CRL1 E3 ubiquitin ligase complex
CAND1 binds cytosolic CRL E3 ubiquitin ligases
COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes
MyrG-DCUN1D3 binds CRL1 E3 ubiquitin ligase complex
Transfer of Ub from E2 to substrate and release of E2
Release of E3 from polyubiquitinated substrate
Polyubiquitination of substrate
Interaction of E3 with substrate and E2-Ub complex
Falcon deep research report for human FBXL12
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FBXL12 is an SCF (SKP1-CUL1-RBX1) substrate-recognition subunit whose biology is best defined by its substrates in different cellular programs, including FANCD2, CaMKI, ALDH3A1/2, and CDKN1B/p27.
"SCF substrate-recognition subunit that drives ubiquitin-dependent remodeling of protein abundance (often proteasomal degradation) for specific substrates in distinct biological programs: replication stress recovery (FANCD2), cell-cycle gating (CaMKI; CDKN1B/p27), and differentiation programs (ALDH3A1/2)."
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Human SCF(FBXL12) promotes CHK1-phosphorylation-dependent proteasomal degradation of chromatin-associated FANCD2 to enable replication-fork recovery and cancer-cell survival under replication stress.
"FANCD2 becomes a substrate of SCF^FBXL12 following **CHK1-dependent phosphorylation**, creating a phosphodegron that triggers FBXL12-dependent turnover, thereby helping clear "chromatin-trapped" FANCD2 at stalled forks and enabling replication restart"
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SCF(FBXL12) targets ALDH3A1 and ALDH3A2 for ubiquitin-dependent degradation, which is essential for trophoblast differentiation during placental development.
"SCF^FBXL12 targets **ALDH3A1 and ALDH3A2** for ubiquitin-dependent degradation and that this is **essential for trophoblast differentiation** and proper placental development"
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SCF(FBXL12) directly K48-polyubiquitinates CDKN1B/p27 (acceptor K165) to license the proliferative burst of thymocyte beta-selection downstream of pre-TCR and Notch signaling.
"SCF-Fbxl12 promoted **K48-linked polyubiquitination** of Cdkn1b, with a key ubiquitination site identified at **K165**; Cdkn1b(K165R) strongly reduced polyubiquitination"
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An SCF complex containing Fbxl12 mediates DNA-damage-induced ubiquitination of Ku80 and its removal from DNA ends (Xenopus extracts, human conservation inferred).
"an **SCF complex containing Fbxl12** was required for **DNA damage-induced Ku80 ubiquitylation**, removal from DNA ends, and subsequent degradation"