Yeast homotypic vacuole fusion requires the Ccz1-Mon1 complex during the tethering/docking stage.
-
S. cerevisiae Ccz1 and Mon1 form a stable vacuole-membrane complex needed for vacuolar fusion and multiple protein-delivery routes to the vacuole.
"Both Mon1 and Ccz1 are required for protein delivery through the Cvt pathway and other vacuole trafficking processes including the CPY and ALP pathways and endocytosis"
-
The Ccz1-Mon1 complex binds to vacuolar membranes and is needed before the tethering/docking stage of homotypic vacuole fusion.
"Ccz1 and Mon1 form a stable protein complex that binds the vacuole membrane."
The Mon1-Ccz1 GEF activates the Rab7 GTPase Ypt7 via a longin-fold-Rab interface and association with PI3P-positive membranes.
-
S. cerevisiae Mon1-Ccz1 requires longin-domain residues from both subunits for GEF activity.
"Mon1–Ccz1 activity requires residues within the predicted longin domains of both subunits"
-
Membrane association strongly increases Mon1-Ccz1 GEF activity toward Ypt7.
"Mon1–Ccz1 GEF complex activates Ypt7 via a common interface, has highest activity on membranes"
Gene Ontology annotation through association of InterPro records with GO terms
Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
UniProtKB Q5A723 flat-file record for Candida albicans MON1
-
Q5A723 is a fungal Mon1 homolog with orthology-projected function in multiple delivery routes to the vacuole.
"FUNCTION: In complex with CCZ1, is required for multiple vacuole"
-
UniProtKB places Candida MON1 at endosomal, prevacuolar, and vacuolar membranes by orthology to characterized Mon1 proteins.
"SUBCELLULAR LOCATION: Endosome, multivesicular body membrane"