Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
Combined Automated Annotation using Multiple IEA Methods
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
UniProtKB Q9Y7T6: curated sequence, features and source attribution
Saccharomyces cerevisiae sec59 cells are deficient in dolichol kinase activity.
Expression and characterization of a human cDNA that complements the temperature-sensitive defect in dolichol kinase activity in the yeast sec59-1 mutant: the enzymatic phosphorylation of dolichol and diacylglycerol are catalyzed by separate CTP-mediated kinase activities in Saccharomyces cerevisiae.
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Sec59 catalyzes CTP-dependent dolichol phosphorylation.
"Dolichol kinase (DK) catalyzes the CTP-mediated phosphorylation of dolichol in
eukaryotic cells, the terminal step in dolichyl monophosphate (Dol-P)
biosynthesis de novo."
UniProtKB P20048: experimentally characterized budding-yeast ortholog