Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Combined Automated Annotation using Multiple IEA Methods
spo0J is required for normal chromosome segregation as well as the initiation of sporulation in Bacillus subtilis.
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Direct primary target evidence with methods/access caveats detailed in spo0J-primary-scope.md.
"These results indicate that Soj inhibits the initiation of sporulation and that Spo0J antagonizes that inhibition."
Bipolar localization of a chromosome partition protein in Bacillus subtilis.
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Direct primary target evidence with methods/access caveats detailed in spo0J-primary-scope.md.
"Spo0J was associated with a region of the nucleoid proximal to the cell pole, both in growing cells dividing symmetrically and in sporulating cells dividing asymmetrically."
Dynamic control of the DNA replication initiation protein DnaA by Soj/ParA.
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Spo0J regulates DNA replication initiation via Soj/ParA
"the ParA ortholog in B. subtilis (Soj) controls the activity of the DNA replication initiator protein DnaA. Subcellular localization of several Soj mutants indicates that Soj acts as a spatially regulated molecular switch, capable of either inhibiting or activating DnaA."
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spo0J disruption leads to increased chromosome origins
"These results suggest that the pleiotropy manifested by chromosomal parABS mutations could be the indirect effects of a primary activity regulating DNA replication initiation."
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Spo0J localizes to the nucleoid
"Subcellular localization of several Soj mutants indicates that Soj acts as a spatially regulated molecular switch"
A network of cis and trans interactions is required for ParB spreading.
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ParB forms nucleoprotein complexes at parS and spreads to adjacent DNA
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Spreading requires cis/trans ParB-ParB interactions
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ParB can bridge DNA segments
Regulation of DNA replication initiation by ParA is independent of parS location in Bacillus subtilis.
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parS consensus sequence is 5'-TGTTCCACGTGAAACA-3'
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B. subtilis has multiple parS sites clustered near oriC
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ParA regulation of DnaA is maintained even when parS is relocated
The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression
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ParB-family proteins are CTP-dependent DNA-sliding clamps
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CTP binding triggers clamp closure and DNA loading
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CTP-binding mutations abolish complex formation
Rules and Exceptions - The Role of Chromosomal ParB in DNA Segregation and Other Cellular Processes.
Deep research on spo0J (ParB) in Bacillus subtilis
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The report supports Spo0J recruitment of chromosome-organizing machinery, corroborated by direct primary experiments.
"ParB:parS recruits SMC–ScpAB to ori region, promoting arm juxtaposition and compaction."
Spo0J reaction and phylogenetic scope check (2026-09-20)
Self-organization of parS centromeres by the ParB CTP hydrolase.
A joint-ParB interface promotes Smc DNA recruitment.
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Direct primary target evidence with methods/access caveats detailed in spo0J-primary-scope.md.
"Using chimeric proteins and chemical cross-linking, we find that ParB directly binds the Smc subunit."
Recruitment of SMC by ParB-parS organizes the origin region and promotes efficient chromosome segregation.
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Direct primary target evidence with methods/access caveats detailed in spo0J-primary-scope.md.
"insertion of parS sites near the replication terminus targets SMC to this position leading to defects in chromosome organization and segregation."
Recruitment of condensin to replication origin regions by ParB/SpoOJ promotes chromosome segregation in B. subtilis.
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Direct primary target evidence with methods/access caveats detailed in spo0J-primary-scope.md.
"Spo0J performs two independent functions: regulation of initiation of DNA replication via Soj and promotion of chromosome segregation by Smc recruitment."