Generated by analyze_partners.py. Partner accessions are read from the gene's own
AARD-goa.tsv; all protein data is fetched from the UniProt REST API at run time.
AARD's entire GO record consists of protein binding annotations from a single
yeast two-hybrid publication. This tests one specific, falsifiable explanation for
that set: coiled-coil bias. Sticky or self-activating preys in Y2H are enriched
for coiled-coil proteins, which associate promiscuously via heptad-repeat surfaces.
If the partners come from unrelated compartments but share coiled-coil
architecture, the assay explains the set better than any function does.
| Partner accessions in the GOA file | 15 |
|---|---|
| Successfully retrieved from UniProt | 15 |
| With a coiled-coil FEATURE | 7 |
| With a coiled-coil feature or keyword | 9 |
| Proportion coiled-coil | 47% |
Background, fetched from UniProt: 2059 of 20431 reviewed human
proteins carry a coiled-coil feature = 10.1%.
Both figures use the coiled-coil FEATURE, on numerator and denominator alike. For completeness, on the more liberal feature-or-keyword criterion (9/15 partners), the matching background is 10.6% (keyword:KW-0175), giving 5.6-fold and P = 4.79e-06. Mixing the two criteria - a keyword numerator against a feature denominator - would overstate the enrichment, so it is not done here.
The enrichment is therefore real rather than assumed, though with n=15 this is
a descriptive statistic and not a controlled test: the relevant comparison would
be against other single-publication prey sets from the same screen, which this
analysis does not attempt.
| Accession | Gene | Coiled-coil segments | Subcellular location |
|---|---|---|---|
| Q4V328 | GRIPAP1 | 4 | Early endosome membrane; Recycling endosome membrane; Cell projection, axon; Cell projection, dendrite; Synapse |
| Q86XR8-3 | CEP57 | 2 | Nucleus; Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
| Q7L2Z9 | CENPQ | 1 | Nucleus; Chromosome, centromere |
| Q2KHM9 | KIAA0753 | 1 | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
| Q16623 | STX1A | 1 | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Synapse, synaptosome; Cell membrane; Secreted |
| P32856-2 | STX2 | 1 | Membrane |
| Q13190 | STX5 | 1 | Endoplasmic reticulum-Golgi intermediate compartment membrane; Golgi apparatus membrane |
| Q2M2I5 | KRT24 | keyword only | — |
| Q7Z3Y8 | KRT27 | keyword only | Cytoplasm |
| P61968 | LMO4 | 0 | — |
| O15481 | MAGEB4 | 0 | Cytoplasm |
| Q96HA8 | NTAQ1 | 0 | Cytoplasm, cytosol; Nucleus |
| Q9UBB9 | TFIP11 | 0 | Cytoplasm; Nucleus |
| Q9BZW7 | TSGA10 | 0 | Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole |
| A5D8V6 | VPS37C | 0 | Late endosome membrane |
A genuine partner set usually concentrates in one or two compartments. UniProt
location strings are hierarchical, so they are collapsed here to their leading
compartment - 20 raw strings reduce to
14 top-level compartments:
AARD belongs to PANTHER PTHR32289 (FAM167 family; InterPro IPR051771).
For a gene this dark, paralogs are the most tractable remaining inference route,
so the family was enumerated:
| Accession | Entry | Organism | FUNCTION |
|---|---|---|---|
| Q0V7M8 | F167A_BOVIN | Bos taurus | — |
| Q5RFZ7 | F167A_DANRE | Danio rerio | — |
| Q9BTA0 | F167B_HUMAN | Homo sapiens | — |
| Q4LEZ3 | AARD_HUMAN | Homo sapiens | — |
| Q96KS9 | F167A_HUMAN | Homo sapiens | — |
| A1L168 | CT202_HUMAN | Homo sapiens | — |
| Q811W1 | AARD_MOUSE | Mus musculus | — |
| P17257 | F167B_MOUSE | Mus musculus | — |
| Q6P1G6 | F167A_MOUSE | Mus musculus | — |
| Q91ZF7 | AARD_RAT | Rattus norvegicus | — |
No member of the FAM167 family has a described function in any organism.
Guilt-by-association across paralogs is therefore unavailable for AARD - not
because it lacks a family, but because the whole family is uncharacterised.
This is consistent with UniProt's PAN-GO line for AARD, which records
0 GO annotations based on evolutionary models.