Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
The Pseudomonas aeruginosa flagellar cap protein, FliD, is responsible for mucin adhesion.
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A fliD insertion mutant is non-adhesive to mucin and is complemented by fliD on a plasmid; purified FliD, but not PilA or FlaG, competitively inhibits mucin adhesion of non-piliated cells.
"A polar chromosomal insertional mutation in the P. aeruginosa fliD gene made this organism nonadhesive to mucin in an in vitro mucin adhesion assay."
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fliD transcription is independent of the flagellar sigma factor sigma-28 and depends on FleQ and RpoN (sigma-54).
"The results of the beta-galactosidase assays suggest that the fliD gene in P. aeruginosa is regulated by the newly described transcriptional regulator FleQ and the alternate sigma factor sigma54 (RpoN)."
Bacterial flagellar capping proteins adopt diverse oligomeric states.
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The PAO1 FliD core (residues 78-405) crystallises as a six-pointed star hexamer corresponding to the distal end of the growing flagellum, and the hexamer is confirmed in solution and by negative-stain EM; full-length FliD also forms hexamers, unlike Salmonella FliD, which is a pentamer.
"Thus, we confirmed that the hexameric assembly of FliD78–405 occurs not only in the crystalline environment but also in solution using negative stain EM class averaging (Tang et al."
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Swimming motility is lost in a PAO1 fliD transposon mutant and restored by PAO1 fliD, but not by Salmonella fliD.
"We found that swimming motility that was lost in the ΔfliD strain was restored in both ΔfliD/fliD1–474 and ΔfliD/fliD1–474(I167C/D253C) complementation strains, similar to our observations in the wildtype Pseudomonas PAO1 strain (Figure 4d)."
Identification of two distinct types of flagellar cap proteins, FliD, in Pseudomonas aeruginosa.
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P. aeruginosa strains carry one of two sequence-divergent fliD types; PAO1 carries the B type, which is the entry reviewed here.
"Identification of two distinct types of flagellar cap proteins, FliD, in Pseudomonas aeruginosa."