Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Human ornithine decarboxylase paralogue (ODCp) is an antizyme inhibitor but not an arginine decarboxylase.
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Establishes that the ODC-like protein is an enzymatically inactive antizyme inhibitor (not a decarboxylase) that binds antizymes AZ1-3 and rescues ODC activity and ODC degradation - the mechanism underlying AZIN1's ODC-activator function.
"ODCp inhibits AZ1 function as efficiently as AZI both in vitro and in vivo"
Antizyme inhibitor 2 (AZIN2/ODCp) stimulates polyamine uptake in mammalian cells.
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Shows that AZIN2 (the AZIN1 paralog), not AZIN1, stimulates polyamine uptake and counteracts antizymes; the polyamine-transport annotation transferred to AZIN1 is derived from this AZIN2 phenotype.
"Transfection of COS7 cells with mouse or human AZIN2, a novel member of the antizyme inhibitor family, recently characterized by our group, markedly stimulated polyamine uptake"
A proteome-scale map of the human interactome network.
Architecture of the human interactome defines protein communities and disease networks.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Structural basis of antizyme-mediated regulation of polyamine homeostasis.
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Crystal structure of AZIN1 in complex with OAZ1 provides the structural basis for how AZIN1 binds antizyme and disrupts the antizyme:ODC interaction, releasing active ODC1.
"Structural basis of antizyme-mediated regulation of polyamine homeostasis"
UniProt entry O14977 (AZIN1_HUMAN)
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AZIN1 is an enzymatically inactive ODC homolog that positively regulates ODC activity and polyamine uptake by competing with ODC for antizyme binding, inhibiting antizyme-dependent ODC degradation and releasing active ODC monomers.
"Antizyme inhibitor (AZI) protein that positively regulates"