PANTHER family review PTHR10177: IBA propagation assessment for cdc13
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2.
-
In cut8 mutants, nuclear destruction of mitotic cyclin Cdc13 and Cut2 is delayed although Cdc13 is still polyubiquitinated, linking Cut8/proteasome localization to cyclin destruction.
"destruction of mitotic cyclin and Cut2 in the nucleus is dramatically delayed, though polyubiquitination of Cdc13 occurs in cut8 mutant."
Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation.
-
The Mfr1-activated APC/C degrades Cdc13 at the end of meiosis II to coordinate meiotic exit with sporulation; persistent Cdc13/Cdc2 activity delays spore formation.
"An mfr1 null mutant completes meiosis II but remains with high levels of cdc13 and cdc2 kinase activity and has considerably delayed spore formation."
In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis.
-
Cdc13-Cdc2 is enriched at the SPB in late G2, accumulates on SPBs and the spindle in prophase/metaphase, and leaves the spindle in anaphase before sister-chromatid separation.
"Cdc2-YFP and cdc13-YFP are highly enriched on the spindle pole body of cells in late G2 or arrested at S phase."
-
In anaphase the complex leaves the spindle prior to sister-chromatid separation and Cdc13 becomes enriched at the nuclear periphery before disappearing (destruction).
"In anaphase, the cdc2p/cdc13p complex leaves the spindle prior to sister chromatid separation"
Cdc2-cyclin B kinase activity links Crb2 and Rqh1-topoisomerase III.
-
Cdc2-cyclin B (Cdc13) kinase activity influences recombinational repair of DSBs in G2 at two stages, with effects on Top3 mediated by the checkpoint protein Crb2.
"the major Schizosaccharomyces pombe CDK, Cdc2-cyclin B, influences recombinational repair of radiation-induced DSBs during the G(2) phase at two distinct stages"
Stable association of mitotic cyclin B/Cdc2 to replication origins prevents endoreduplication.
-
Cdc13/Cdc2 associates with ORC-bound replication origins in vivo, imposing the dependence of S phase on an intervening mitosis and preventing re-replication.
"the mitotic B type cyclin Cdc13/Cdc2 kinase associates with replication origins in vivo. This association is dependent on the origin recognition complex (ORC)"
Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p.
-
Mes1 blocks APC/C-dependent degradation of Cdc13 at anaphase I, preserving MPF activity required for meiosis II.
"Mes1p is a factor that suppresses the degradation of cyclin Cdc13p at anaphase I"
Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function.
-
Thr167 phosphorylation of Cdc2 is required for kinase function and is implicated in association of Cdc2 with cyclin B (Cdc13).
"Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function."
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Distinct nuclear and spindle pole body population of cyclin-cdc2 in fission yeast.
-
Two spatially distinct nuclear populations of Cdc13 cyclin exist, one associated with the mitotic spindle poles, both co-localizing with Cdc2.
"the presence of two spatially distinct cdc13 cyclin populations in the nucleus of S. pombe, one of which is associated with the mitotic spindle poles"
Phospho-regulated interaction between kinesin-6 Klp9p and microtubule bundler Ase1p promotes spindle elongation.
-
Cdc2 (Cdc13-CDK) phosphorylates and Clp1 dephosphorylates Klp9 and Ase1 to control midzone Klp9-Ase1 interaction and anaphase B spindle elongation.
"The cyclin-dependent kinase cdc2p phosphorylates and its antagonist phosphatase clp1p dephosphorylates klp9p and ase1p to control the position and timing of klp9p-ase1p interaction."
Phosphorylation of the CPC by Cdk1 promotes chromosome bi-orientation.
A role for metaphase spindle elongation forces in correction of merotelic kinetochore attachments.
-
Cdk1 (Cdc2-Cdc13) regulates metaphase spindle elongation forces to correct merotelic kinetochore attachments.
"A role for metaphase spindle elongation forces in correction of merotelic kinetochore attachments."
Fission yeast cyclin: subcellular localisation and cell cycle regulation.
-
Cdc13 and Cdc2 are nuclear proteins; Cdc13 is required to localize Cdc2 to the nucleus and behaves as a classic cyclin (destroyed at mitosis).
"cdc13 therefore appears to be required to localise cdc2 to the nucleus but not vice versa."
The fission yeast cdc2/cdc13/suc1 protein kinase: regulation of catalytic activity and nuclear localization.
-
Cdc13 and Cdc2 form a stable kinase-active complex; the Cdc13 cyclin regulates both the catalytic properties and the localization of the Cdc2 kinase.
"The products of the cdc13+ and cdc2+ genes form a stable complex that displays protein kinase activity in vitro."
A single cyclin-CDK complex is sufficient for both mitotic and meiotic progression in fission yeast.
-
A single Cdc13-Cdc2 complex can drive both the mitotic and the meiotic cell cycle; Cdc13 is required for meiosis I and II.
"Cdc13 is required for meiosis I and II, and is not required for premeiotic S-phase unless this function can be achieved by very low Cdc13 protein levels"
Two Distinct Cdc2 Pools Regulate Cell Cycle Progression and the DNA Damage Response in the Fission Yeast S.pombe.
-
Two distinct Cdc2 (Cdc13-CDK) pools regulate cell-cycle progression and the DNA damage response.
"Two Distinct Cdc2 Pools Regulate Cell Cycle Progression and the DNA Damage Response in the Fission Yeast S.pombe."
Cloning and sequencing of the cyclin-related cdc13+ gene and a cytological study of its role in fission yeast mitosis.
The telomere bouquet facilitates meiotic prophase progression and exit in fission yeast.
The Hydrophobic Patch Directs Cyclin B to Centrosomes to Promote Global CDK Phosphorylation at Mitosis.
-
The Cdc13 hydrophobic patch targets the cyclin to the SPB; this localization is required for mitotic entry and for full phosphorylation of SPB/cytoplasmic mitotic CDK substrates, but not for S phase.
"the hydrophobic patch targets Cdc13 to the yeast centrosome equivalent, the spindle pole body (SPB), and disruption of this motif prevents both centrosomal localization of Cdc13 and the onset of mitosis but does not prevent S phase"
Acute Heat Stress Leads to Reversible Aggregation of Nuclear Proteins into Nucleolar Rings in Fission Yeast.
-
Acute heat stress reversibly aggregates nuclear/nucleolar proteins, including cell-cycle regulators, into nucleolar rings at the nucleolar periphery.
"NuRs sequester essential factors required for nuclear mRNA metabolism and nuclear pore complex function, as well as cell-cycle regulators."
CDK actively contributes to establishment of the stationary phase state in fission yeast.
-
Upon stationary-phase entry / glucose depletion, Cdc2 (CDK) accumulates in the nucleolus and actively contributes to the stationary-phase state.
"Cdc2 accumulates in the nucleolus."
Cyclin B (p56cdc13) localization in the yeast Schizosaccharomyces pombe: an ultrastructural and immunocytochemical study.
-
EM/immunocytochemistry shows a major cytoplasmic pool of p56cdc13 in interphase in addition to nuclear localization, with relocalization to the nucleus in mitosis.
"evidencing cytoplasmic localization of p56cdc13, in addition to the nuclear localization previously observed"
p25rum1 promotes proteolysis of the mitotic B-cyclin p56cdc13 during G1 of the fission yeast cell cycle.
-
The CDK inhibitor Rum1 associates with the Cdc2-Cdc13 complex during G1 and is required for the rapid proteolysis of Cdc13.
"p25rum1 associates with the CDK p34cdc2/p56cdc13 during G1 in normally cycling cells and is required for the rapid proteolysis of p56cdc13"
The Cdk inhibitors p25rum1 and p40SIC1 are functional homologues that play similar roles in the regulation of the cell cycle in fission and budding yeast.
-
Rum1 (and its budding-yeast homolog Sic1) are specific inhibitors of p34cdc2 kinase complexes with B-type cyclins such as Cdc13.
"p25rum1 and p40SIC1 are specific inhibitors of p34(cdc2/CDC28) kinase complexes with B-type cyclins"
Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast.
-
Cyclin B (Cdc13) proteolysis and the CDK inhibitor Rum1 are required for pheromone-induced G1 arrest.
"Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast."