Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry for PP_1703 (Q88M71)
OpenScientist functional annotation report for PSEPK PP_1703 (Q88M71)
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The completed report identifies a fused diflavin assimilatory nitrate reductase architecture, distinguishes it from NapA and CysJ, and records the absence of direct Q88M71 enzymology as a limitation.
"The correct primary function is **cytoplasmic assimilatory nitrate reduction**."
Inactivation of gltB abolishes expression of the assimilatory nitrate reductase gene (nasB) in Pseudomonas putida KT2442.
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Closely related KT2442 genetics directly identifies nasB as an assimilatory nitrate reductase gene induced during ammonium deficiency.
"is demonstrated to bear the transposon within the assimilatory nitrate reductase gene (nasB) of P. putida KT2442"
A recently evolved diflavin-containing monomeric nitrate reductase is responsible for highly efficient bacterial nitrate assimilation.
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Biochemical work on NasN establishes that a molybdopterin catalytic domain fused to an FMN-FAD/NAD-binding module can form a monomeric NADPH-dependent assimilatory nitrate reductase.
"NasN is an NADPH-dependent, diflavin-containing monomeric enzyme composed of a canonical molybdopterin cofactor-binding catalytic domain and an FMN-FAD/NAD-binding, electron-receiving/transferring domain"
Assimilatory nitrate reductase from Acinetobacter calcoaceticus.
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Comparative bacterial biochemistry establishes a soluble assimilatory nitrate reductase that reduces nitrate to nitrite with NAD(P)H supplied through diaphorases.
"A soluble nitrate reductase from the bacterium Acinetobacter calcoaceticus grown on nitrate has been characterized."
A Nitrate-Blind P. putida Strain Boosts PHA Production in a Synthetic Mixed Culture.
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The introduction cites PP_1703 and nirBD as the assigned nitrate- and nitrite-reductase genes in KT2440 nitrate assimilation.
"the assimilating nitrate and nitrite reductases encoded by PP_1703 and nirBD (PP_1705, PP_1706)"
Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing.
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Native KT2440 pooled-mutant fitness supports nitrate uptake by PP_2092; the nitrate-condition fitness score is −2.6.
"We were also able to identify a distinct nitrate transporter PP_2092 (−2.6)."
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Absence of a significant nitrite-condition phenotype does not exclude redundant or conditional nitrite transport.
"However, no significant phenotype could be detected for this gene in the nitrite condition, suggesting that it may be specific to nitrate."
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The paper reports NarB and combined NirDB pathway phenotypes, without assigning the displayed combined NirDB score to each individual subunit.
"The enzymes associated with the initial steps of the assimilatory nitrogen system in P. putida are the reductases NarB and NirDB, for which we see significant growth phenotypes"
Inactivation of gltB Abolishes Expression of the Assimilatory Nitrate Reductase Gene (nasB) in Pseudomonas putida KT2442
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KT2442 native gene disruption and complementation identify an assimilatory nitrate-reductase catalytic gene.
"In conclusion, these results clearly demonstrate that P. putida N25 bears the mini-Tn5 luxAB transposon within the assimilatory nitrate reductase."
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The cloned 2175-bp gene restores nitrate-supported growth; it corresponds to a catalytic fragment of the modern fused annotation.
"while cells of both strains harboring pAA2 were able to grow"
Prokaryotic Nitrate Reduction: Molecular Properties and Functional Distinction among Bacterial Nitrate Reductases
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The assimilatory Nas nitrate reductases are the cytoplasmic class, distinct from membrane-bound Nar and periplasmic Nap.
"comprising the cytoplasmic assimilatory (Nas), membrane-bound respiratory (Nar), and periplasmic dissimilatory (Nap) nitrate reductases"
file:PSEPK/PP_1703/PP_1703-source-analysis/README.md