GO_REF:0000024

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000041

Gene Ontology annotation based on UniPathway vocabulary mapping

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

PMID:15678131

The anaphase-promoting complex: a key factor in the regulation of cell cycle.

PMID:16364912

Localization of the coactivator Cdh1 and the cullin subunit Apc2 in a cryo-electron microscopy model of vertebrate APC/C.

APC/C is a ubiquitin ligase with essential mitotic, meiotic, and G1 functions.
Apc2 and Apc11 form the cullin/RING side of APC/C where substrates are ubiquitinated.

PMID:11739784

APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex.

Human APC2 with APC11 reconstitutes a minimal APC/C ubiquitin ligase module that ubiquitinates securin and cyclin B1 with Ubc4 or UbcH10, while lacking substrate specificity alone.

PMID:18485873

Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex.

Human APC/C assembles K11-linked ubiquitin chains that support substrate degradation.

PMID:23708001

Emi1 preferentially inhibits ubiquitin chain elongation by the anaphase-promoting complex.

Emi1 inhibits APC/C chain elongation in part by blocking Ube2S binding to the APC cullin subunit.

PMID:26083744

Atomic structure of the APC/C and its mechanism of protein ubiquitination.

Cryo-EM structures define the architecture of APC/C subunits and the catalytic module.
The Apc2-Apc11 catalytic module provides structural support for a scaffold interpretation of ANAPC2.

PMID:27120157

Molecular mechanism of APC/C activation by mitotic phosphorylation.

APC/C regulates ubiquitin-dependent proteolysis of cell-cycle proteins during mitosis and G1 entry.

PMID:29033132

Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control.

K11/K48 branched ubiquitin chains occur on mitotic regulators and proteostasis substrates and promote rapid proteasomal clearance.

PMID:32296183

A reference map of the human binary protein interactome.

HuRI provides high-throughput binary protein-interaction context; individual PPIs need functional interpretation.

PMID:37735619

Time-resolved cryo-EM (TR-EM) analysis of substrate polyubiquitination by the RING E3 anaphase-promoting complex/cyclosome (APC/C).

Time-resolved cryo-EM of active human APC/C shows the priming E2 UBE2C is clasped by the ANAPC11 RING and the ANAPC2 (APC2) WHB region, while the elongation E2 UBE2S C-terminal peptide binds a groove formed by ANAPC2-ANAPC4, supporting a direct E2-docking role for ANAPC2.
The ANAPC2-ANAPC11 catalytic arm samples autoinhibited "CRL down" and active "CRL up" states, and UBE2S allosterically stabilizes the "CRL up" state to potentiate processive substrate polyubiquitination.

PMID:39567505

Cryo-EM structures of apo-APC/C and APC/C(CDH1:EMI1) complexes provide insights into APC/C regulation.

High-resolution cryo-EM identified a previously unreported zinc-binding module in ANAPC2 (APC2) that confers structural stability to the APC/C, with zinc ions confirmed experimentally.
The structures resolve EMI1 inhibitor and CDH1 contacts on the APC/C catalytic platform that includes ANAPC2.

PMID:36548081

The UBE2C/CDH1/DEPTOR axis is an oncogene and tumor suppressor cascade in lung cancer cells.

APC/C-CDH1 with the E2 UBE2C ubiquitylates and degrades DEPTOR to activate mTORC signaling, and knockdown of APC2 or CDH1 increased DEPTOR protein, indicating ANAPC2-containing APC/C is required for DEPTOR turnover in lung cancer cells.

Reactome:R-HSA-141423

Binding of the MCC complex to the APC/C complex

Reactome:R-HSA-174057

Multiubiquitination of APC/C-associated Cdh1

Reactome:R-HSA-174058

Degradation of multiubiquitinated Cdh1

Reactome:R-HSA-174070

Association of Cdh1 with the APC/C

Reactome:R-HSA-174079

Phosphorylation of Cdh1 by Cyclin A:Cdk2

Reactome:R-HSA-174088

Association of cell cycle proteins with the APC/C:Cdh1 complex

Reactome:R-HSA-174104

Ubiquitination of Cyclin A by APC/C:Cdc20 complex

Reactome:R-HSA-174105

Degradation of multiubiquitinated cell cycle proteins

Reactome:R-HSA-174119

Free APC/C phosphorylated by Plk1

Reactome:R-HSA-174120

Association of Cyclin B:Cdc2 with Cdc20:APC/C complex

Reactome:R-HSA-174121

Association of Securin with Cdc20:APC/C complex

Reactome:R-HSA-174132

Free APC/C phosphorylated by Cyclin B:Cdc2

Reactome:R-HSA-174139

Dissociation of phospho-Cdh1 from the APC/C complex

Reactome:R-HSA-174144

Ubiquitination of Securin by phospho-APC/C:Cdc20 complex

Reactome:R-HSA-174157

Degradation of multiubiquitinated Cyclin B

Reactome:R-HSA-174171

Association of Cyclin A with the APC/C

Reactome:R-HSA-174195

Ubiquitination of cell cycle proteins targeted by the APC/C:Cdh1complex

Reactome:R-HSA-174202

Degradation of multiubiquitinated Securin

Reactome:R-HSA-174224

Dissociation of Cdc20 from APC/C complex

Reactome:R-HSA-174227

Ubiquitination of Cyclin B by phospho-APC/C:Cdc20 complex

Reactome:R-HSA-174238

Activation of APC/C:Cdc20 by dissociation of Cdc20:phospho-APC/C from Cdc20:phospho-APC/C:Mad2:Bub3:BubR1

Reactome:R-HSA-174255

Degradation multiubiquitinated Cyclin A

Reactome:R-HSA-179410

Association of Nek2A with MCC:APC/C

Reactome:R-HSA-179417

Multiubiquitination of Nek2A

Reactome:R-HSA-179421

Degradation of multiubiquitinated Nek2A

Reactome:R-HSA-188191

APC/C:Cdh1-mediated degradation of Skp2

Reactome:R-HSA-188371

Association of Cyclin A:Cdk2 with Cdh1

Reactome:R-HSA-3788705

CDKN1A (p21) prevents phosphorylation of Cdh1 by Cyclin A:Cdk2

Reactome:R-HSA-3788708

CDKN1A (p21) prevents association of Cyclin A:Cdk2 with Cdh1

Reactome:R-HSA-3788724

Cdh1:APC/C ubiquitinates EHMT1 and EHMT2

Reactome:R-HSA-3788725

Cdh1:APC/C complex binds EHMT1:EHMT2

Reactome:R-HSA-68712

The geminin component of geminin:Cdt1 complexes is ubiquitinated, releasing Cdt1

Reactome:R-HSA-69015

Cytoplasmic phosphorylated Cdc6 is ubiquitinated by the anaphase-promoting complex

Reactome:R-HSA-9686969

APC/C:Cdh1 polyubiquitinates SKP2

Reactome:R-HSA-9686980

RB1 recruits APC/C:Cdh1 complex to SKP2

Reactome:R-HSA-9687377

Defective RB1 does not form a complex with SKP2 and FZR1

file:human/ANAPC2/ANAPC2-uniprot.txt

UniProtKB record for human ANAPC2

UniProt describes ANAPC2 as the cullin-family APC/C subunit that works with ANAPC11 in the APC/C catalytic component.

file:human/ANAPC2/ANAPC2-notes.md

ANAPC2 review notes and PN projection assessment

Falcon deep research timed out and the configured Perplexity fallback failed with quota error.
The PN scaffold projection was accepted only after gene-level review of ANAPC2 structural evidence.

file:projects/PROTEOSTASIS/reports/pn_projection/pn_projected_gene_go_summary.tsv

PN projected gene-GO summary report

ANAPC2 already has exact APC/C complex coverage and entailed ubiquitin ligase complex coverage in GOA.

file:projects/PROTEOSTASIS/reports/pn_projection/pn_projected_candidate_additions.tsv

PN projected candidate additions report

ANAPC2 has a new-to-GOA PN candidate for GO:0160072 ubiquitin ligase complex scaffold activity.