TRAPPC12 PN Consistency Notes
- Generated: 2026-06-18
- Project: PROTEOSTASIS
- Scope: PN consistency rereview against local AIGR review and available deep-research artifacts
- UniProt: Q8WVT3
- AIGR review status: COMPLETE
- Review batch: proteostasis-pr-1217 (PR 1217)
- Batch change status: added
Source Files Checked
Deep Research Files
- No
*-deep-research*.md file found in this gene directory.
AIGR Review Snapshot
- Description: TRAPPC12/TRAMM/TTC-15 is a moonlighting metazoan TRAPP/TRAPPIII-associated protein. In interphase it participates in TRAPP-dependent early secretory traffic between ER, ERGIC, and Golgi. During mitosis it leaves the TRAPP context and supports chromosome congression, kinetochore stability, and CENP-E recruitment. Its shared cellular roles are TRAPP/TRAPPII/TRAPPIII complex membership and TRAPP/RAB1 trafficking; direct TRAPPC12-specific autophagy evidence is limited.
- Existing/core annotation action counts: ACCEPT: 18; KEEP_AS_NON_CORE: 1; MARK_AS_OVER_ANNOTATED: 3; MODIFY: 3
PN Consistency Summary
- Consistency: Consistent. Notes and YAML agree on two directly-supported tracks — interphase TRAPP/TRAPPIII early-secretory traffic, and a moonlighting mitotic kinetochore/CENP-E role (PMID:25918224) — and explicitly decline a TRAPPC12-specific autophagy annotation ("I am not adding a direct autophagy annotation… autophagy is represented by TRAPPIII/RAB1 context only"). This matches the PN complex-only mapping. No contradictions.
- PN story / NEW pressure: PN restricts to GO:0030008 membership; unlike TRAPPC11, TRAPPC12 has no direct gene-specific autophagy evidence, so the review correctly does not mint an autophagy BP (
proposed_new_terms: []). GO:0030008 is verified real and already in GOA. The mitotic moonlighting functions (GO:0051310, GO:0090234, GO:1905342) are outside the PN scope and correctly flagged as non-PN-propagation targets. Conclude: already captured; no NEW warranted — good asymmetry vs TRAPPC11.
- Evidence alignment: Overlap on TRAPP: PN cites PMID:27066478 + Frontiers review = review core. Review enriches with PMID:21525244 (TRAPP ID), 28777934 (CDG/encephalopathy), 25918224 (mitosis) — none in PN, all consistent. (Notes flag a spurious PANTHER PTHR21581 "D-Ala-D-Ala carboxypeptidase" artifact, correctly not used.)
- Verdict: Fully consistent; correctly conservative on autophagy. No edits needed.
Full Consistency Review
- UniProt: Q8WVT3 (TRAMM/TTC-15) · batch: proteostasis-pr-1217 · review status: COMPLETE
- PN placement:
Autophagy-Lysosome Pathway → Autophagophore initiation and elongation → Autophagy component recruitment to autophagophore → TRAPP complex component (1 row, ALP) ; PN-node mapping: leaf type=mapped/ok_for_propagation→GO:0030008 TRAPP complex; group=no_mapping; class=context_only/too_broad→GO:0016236 macroautophagy; branch=no_mapping. Projects GO:0030008 (already_in_goa_exact).
- Consistency: Consistent. Notes and YAML agree on two directly-supported tracks — interphase TRAPP/TRAPPIII early-secretory traffic, and a moonlighting mitotic kinetochore/CENP-E role (PMID:25918224) — and explicitly decline a TRAPPC12-specific autophagy annotation ("I am not adding a direct autophagy annotation… autophagy is represented by TRAPPIII/RAB1 context only"). This matches the PN complex-only mapping. No contradictions.
- PN story / NEW pressure: PN restricts to GO:0030008 membership; unlike TRAPPC11, TRAPPC12 has no direct gene-specific autophagy evidence, so the review correctly does not mint an autophagy BP (
proposed_new_terms: []). GO:0030008 is verified real and already in GOA. The mitotic moonlighting functions (GO:0051310, GO:0090234, GO:1905342) are outside the PN scope and correctly flagged as non-PN-propagation targets. Conclude: already captured; no NEW warranted — good asymmetry vs TRAPPC11.
- Mapping strategy: Gene does not change the node (GO:0030008 correct, macroautophagy correctly held at
context_only). The mitotic functions are appropriately excluded from the shared TRAPP bucket.
- Evidence alignment: Overlap on TRAPP: PN cites PMID:27066478 + Frontiers review = review core. Review enriches with PMID:21525244 (TRAPP ID), 28777934 (CDG/encephalopathy), 25918224 (mitosis) — none in PN, all consistent. (Notes flag a spurious PANTHER PTHR21581 "D-Ala-D-Ala carboxypeptidase" artifact, correctly not used.)
- Verdict: Fully consistent; correctly conservative on autophagy. No edits needed.
PN Dossier Context
- review_batch: proteostasis-pr-1217
- review_yaml: genes/human/TRAPPC12/TRAPPC12-ai-review.yaml
- PN workbook rows: 1
PN row 1: Autophagy-Lysosome Pathway | Autophagophore initiation and elongation | Autophagy component recruitment to autophagophore | TRAPP complex component
- UniProt: Q8WVT3
- In branches: ALP
- Notes: TRAPP III complex, specific subunit. The TRAPP complex serves as a GEF for RAB1. Involved in ATG9 and ATG2 trafficking
- PN references (titles):
- Membrane Trafficking in Autophagy - ScienceDirect
- Frontiers | TRAPP Complexes in Secretion and Autophagy | Cell and Developmental Biology (frontiersin.org)
- PN-node mapping records (path + ancestors):
- [type] Autophagy-Lysosome Pathway|Autophagophore initiation and elongation|Autophagy component recruitment to autophagophore|TRAPP complex component
status=mapped scope=ok_for_propagation_to_go GO=[GO:0030008 TRAPP complex]
rationale: This PN leaf is a curated component bucket for TRAPP subunits used in autophagophore recruitment. The matching GO cellular-component term is TRAPP complex, and the member genes already converge strongly on that assignment in existing GOA.
- [group] Autophagy-Lysosome Pathway|Autophagophore initiation and elongation|Autophagy component recruitment to autophagophore
status=no_mapping scope= GO=[]
rationale: Reviewed as a broad PN taxonomy container. The descendants mix components, regulators, context labels, and mechanistic leaves, so propagation should come only from narrower curated nodes.
- [class] Autophagy-Lysosome Pathway|Autophagophore initiation and elongation
status=context_only scope=too_broad_to_propagate GO=[GO:0016236 macroautophagy]
rationale: This class is a real macroautophagy context, but its descendants include core factors, component buckets, upstream modulators, localization roles, and residual categories. Projecting generic macroautophagy from this ancestor creates TRAPP-like overpropagation, so candidate GO annotations must come from narrower curated nodes.
- [branch] Autophagy-Lysosome Pathway
status=no_mapping scope= GO=[]
rationale: Reviewed as the top-level PN branch. It is a project taxonomy umbrella rather than a direct GO assertion; all propagation must come from manually curated child nodes.
Projected GO annotations (1)
- GO:0030008 TRAPP complex | scope=ok_for_propagation_to_go | goa_status=already_in_goa_exact | from=Autophagy-Lysosome Pathway|Autophagophore initiation and elongation|Autophagy component recruitment to autophagophore|TRAPP complex component
Note
This file is generated from the current PROTEOSTASIS phase-1 dossier and local gene-review artifacts. Edit the source review, PN mapping, or dossier rather than this generated note when correcting the underlying curation.