Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Combined Automated Annotation using Multiple IEA Methods
Apicobasal domain identities of expanding tubular membranes depend on glycosphingolipid biosynthesis.
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sptl-1 RNAi causes conversion of intestinal apicobasal polarity with ectopic lateral lumens at L1 arrest; phenocopied by sptl-2;sptl-3 double RNAi and by SPT/CerS inhibitors.
"Sphingolipid's role in polarity was, however, confirmed via sptl-1(RNAi) phenocopy by: double knockdowns of sptl-2 and sptl-3, two additional C.elegans SPTs (Fig.S6e)"
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sptl-1 RNAi intestines show vesicle paucity and distended ER and Golgi membranes.
"sptl-1(RNAi) intestinal sections revealed vesicle paucity, along with distended endoplasmic reticular (ER) and Golgi membranes"
Monomethyl branched-chain fatty acids play an essential role in Caenorhabditis elegans development.
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Early identification of C23H3.4 (then spt-1) as the C. elegans SPT subunit 1 homolog; used as an RNAi comparator in expression profiling.
"The spt-1 (C23H3.4) gene encodes a predicted C. elegans homolog of serine-palmitoyl transferase subunit 1."
A novel sphingolipid-TORC1 pathway critically promotes postembryonic development in Caenorhabditis elegans.
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sptl-1 is required for d17iso-sphinganine synthesis; d17iso-sphinganine supplementation suppresses sptl-1(RNAi) L1 arrest.
"supplementation with d17iso-SPA, but not mmBCFAs, also suppressed the L1 arrest phenotype caused by feeding RNAi of sptl-1"
Sphingolipid profiling reveals differential functions of sphingolipid biosynthesis isozymes of Caenorhabditis elegans.
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sptl-1 RNAi lowers nearly all 47 quantified Cer, GlcCer and SM species in young adults.
"RNAi targeting the first key enzyme (SPT) in the de novo sphingolipid biosynthesis pathway caused a decrease of nearly all sphingolipids in Caenorhabditis elegans."
A Model of Hereditary Sensory and Autonomic Neuropathy Type 1 Reveals a Role of Glycosphingolipids in Neuronal Polarity.
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CRISPR SPTL-1(C121W), equivalent to human SPTLC1 C133W, behaves as loss of function (homozygous) and dominant-negative (overexpression), reducing glucosylceramide.
"Overexpression of SPTL-1(C121W) led to similar epithelial and neuronal defects and to reduced levels of complex sphingolipids"
Sphingosine kinase SPHK-1 maintains sphingolipid metabolism to protect lysosome membrane integrity in C. elegans.
Pairwise alignment of SPTL-1 with human SPTLC1 and SPTLC2
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SPTL-1 is SPTLC1-like and lacks the PLP Schiff-base lysine.
"At the position aligned to the SPTLC2 PLP Schiff-base lysine (K379), SPTL-1 has Asn297, not Lys."