JEM1 review notes

2026-10-01 IBA/GOA refresh

• Refreshed JEM1 against current GOA with just fetch-gene yeast JEM1 --force. The current export adds direct UniProt EXP localization rows for GO:0005789 endoplasmic reticulum membrane and GO:0031965 nuclear membrane and no longer exports SGD's older GO:0051082 unfolded protein binding IMP row, which remains in the review as retired: true.
• The current PTHR44140 PAINT slice places all four live JEM1 IBA rows at PANTHER:PTN001941043: GO:0051087 protein-folding chaperone binding, GO:0005783 endoplasmic reticulum, GO:0034975 protein folding in endoplasmic reticulum, and GO:0051787 misfolded protein binding. The GO:0034975 process node uses budding-yeast JEM1 itself as a descendant seed, which is valid PAINT target self-evidence rather than circularity.
• PMID:9148890 is abstract-only in the cache but directly supports ER localization and the DnaJ/Kar2 axis: "An epitope-tagged version of the protein was anchored in the endoplasmic reticulum (ER) membrane and its J-domain faced the ER lumen" and "JEM1p likely assists the functions of BiP, Hsp70 in the ER, including karyogamy." PMID:9148890
• PMID:11381090 is full text in the cache and supports retaining the inherited ERAD substrate-handling terms. The authors report that Jem1p and Scj1p are involved in ERAD of CPY* and deltaGpalphaF and conclude that "BiP cooperates with the soluble J domain proteins in the ER, Jem1p and Scj1p, to facilitate the export of ERAD substrates to the cytosol by preventing their aggregation in the ER lumen." PMID:11381090
• PMID:9817751 supports the partially overlapping Jem1/Scj1 contribution to Kar2-mediated ER folding under stress, including the statement that "Jem1p and Scj1p appear to have partially overlapping functions as cofactors for Kar2p." PMID:9817751
• The Mps3/Nep98 IntAct row from PMID:12493774 is credible biology, but the only GO assertion is generic GO:0005515 protein binding. Because the accessible abstract only establishes a two-hybrid screen that "identified Nep98p" as a Jem1 partner, there is no exact, more informative MF replacement; per project policy the row is better removed as uninformative than retained as over-annotated. PMID:12493774
• PMID:15282802 remains abstract-only and does not name Jem1/KAR8 in the abstract, but the refreshed UniProt record cites it with ECO:0000269 support for JEM1 Nucleus membrane. This supports keeping the current UniProt EXP GO:0031965, UniProtKB-SubCell GO:0031965, and SGD IDA GO:0042175 nuclear-membrane/network rows as contextual localization evidence. [file:yeast/JEM1/JEM1-uniprot.txt]