THI4 notes (P32318, YGR144W)

• Suicide ADP-thiazole synthase (EC 2.4.2.60): NAD+ + Gly -> ADT, S from Cys205 -> dehydroalanine; Fe(II) dependent PMID:22031445.
• Only thiazole-forming gene product in eukaryotes PMID:22031445.
• thi4 auxotrophy rescued by hydroxyethylthiazole PMID:7941734; cytosolic by fractionation PMID:15544818; nucleus too [UniProt:P32318 citing PMID:16850348].
• YeastPathways also assigns Thi4 to ADT hydrolysis (ADT + H2O -> AMP + thiazole-P); no supporting evidence found in cached literature; the enzyme for that step in yeast appears unresolved.
• Review: 16 ACCEPT, 1 MODIFY (pentosyltransferase -> GO:0160205), 5 KEEP_AS_NON_CORE (iron ion binding x2, ferrous iron binding, nucleus x2).