Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
Characterization and analysis of a novel diguanylate cyclase PA0847 from Pseudomonas aeruginosa PAO1.
-
Purified PAS-GGDEF of PA0847 synthesises c-di-GMP in vitro; GGDEF alone is nearly inactive.
"Although isolated GGDEF domain showed only residual DGC activity (data not shown), PAS-GGDEF duel domain efficiently catalyzed the formation of c-di-GMP in vitro."
-
The deletion mutant is hypermotile.
"Compared with wild type strain, PA0847 mutant bacteria displayed significantly increased swimming and swarming motilities (Figure 1A and B)."
Evaluation and characterization of the predicted diguanylate cyclase-encoding genes in Pseudomonas aeruginosa.
-
The PA0847 deletion lowers cellular c-di-GMP.
"Among DGC mutants, ∆PA0169, ∆PA0847, ∆PA1120 (yfiN), ∆PA3702 (wspR), and ∆PA4332 produced significantly less c‐di‐GMP compared to wild type."