Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry Q88N74 for Pseudomonas putida KT2440 ddlB
-
UniProt assigns direct D-Ala-D-Ala synthesis.
"Reaction=2 D-alanine + ATP = D-alanyl-D-alanine + ADP + phosphate +"
-
UniProt assigns magnesium or manganese cofactors.
"Note=Binds 2 magnesium or manganese ions per subunit."
-
UniProt places DdlB in peptidoglycan synthesis.
"PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis."
OpenScientist deep-research report for PSEPK ddlB
-
The report identifies DdlB as the ATP-dependent enzyme that forms D-Ala-D-Ala for MurF-dependent pentapeptide assembly.
"DdlB provides the D-Ala-D-Ala dipeptide that the ligase **MurF** adds to UDP-MurNAc-tripeptide, completing **UDP-MurNAc-pentapeptide**"
-
Direct biochemical characterization of the KT2440 target remains unavailable.
"**Direct experimental evidence for PP_1339 (Q88N74) itself is lacking**; no *P. putida*-specific enzymology, structure, or knockout was found."