Purification and characterization of dihydroorotase from Pseudomonas putida.

Purified P. putida dihydroorotase is a reversible, zinc-responsive homodimer with strict activity on dihydro-L-orotate and its methyl ester.

"hydrolyzed dihydro-L-orotate and its methyl ester, and the reactions were"

Gene Ontology annotation through association of InterPro records with GO terms

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

GO_REF:0000118

TreeGrafter-generated GO annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/pyrC/pyrC-uniprot.txt

UniProtKB reviewed entry for Pseudomonas putida KT2440 PyrC

UniProt defines zinc-dependent dihydroorotase chemistry and de novo UMP pathway placement.

file:PSEPK/pyrC/pyrC-deep-research-openscientist.md

OpenScientist deep research report on pyrC (Q88NW7)

The report corroborates the Q88NW7 dihydroorotase assignment and identifies the direct species-level enzymology while preserving its strain-provenance caveat.

"The gene **pyrC** (ordered locus **PP_1086**; UniProt **Q88NW7**; *PYRC_PSEPK*) of *Pseudomonas putida* KT2440 encodes **dihydroorotase (DHOase; EC 3.5.2.3)**"