Gene Ontology annotation through association of InterPro records with GO terms
The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins.
A proteomic strategy for global analysis of plant protein complexes.
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Two homologous INDOLE-3-ACETAMIDE (IAM) HYDROLASE genes are required for the auxin effects of IAM in Arabidopsis.
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IAMH1 (At4g37550) was isolated as an IAM-insensitive mutant and confirmed by a T-DNA allele and complementation.
"We renamed"
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IAMH1 is broadly expressed and the IAMH1-GFP fusion is excluded from the nucleus.
"At seedling stage, the GUS expression was broadly distributed in cotyledons, true leaves, and root tips"
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The iamh1 iamh2 double mutants suppress iaaM-induced auxin overproduction but show no developmental defects under normal conditions.
"The iamh1 iamh2 double mutants did not display obvious developmental defects under our normal growth conditions, suggesting that auxin synthesized from the IAM pathway may not play critical roles in Arabidopsis development."
Structural variation in the IAMH2 3' UTR underlies hypocotyl elongation during light adaptation in Arabidopsis thaliana.
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IAMH1 is upregulated in iamh2 mutants under low light (partial compensation), but only IAMH2 is light-responsive in Col-0, and the compensation does not restore hypocotyl elongation.
"IAMH1 was upregulated in the iamh2 mutant under low light (Figure S8A), indicating partial compensation."
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The authors conclude that IAMH2 is non-redundant with IAMH1 for low-light hypocotyl elongation.
"However, only IAMH2 was light‐responsive in Col‐0 (Figure S8B), and this compensation did not restore hypocotyl elongation, suggesting that IAMH2 plays a non‐redundant role in this context."
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The paper cites work questioning whether the IAM pathway contributes to auxin biosynthesis under standard conditions.
"recent studies have questioned whether this pathway contributes to auxin biosynthesis under standard conditions"
Identification of a novel link connecting indole-3-acetamide with abscisic acid biosynthesis and signaling.
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IAM-triggered root growth inhibition depends on ABA biosynthesis (ABA3) and signalling, so IAM may act as a signalling molecule beyond its role as an auxin precursor.
"These results imply that IAM may have a function beyond its role as an intermediate in a secondary pathway of auxin biosynthesis, potentially acting as an independent signaling molecule"
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Describes the IAM pathway as contributing to IAA biosynthesis only under specific conditions.
"the indole‐3‐acetamide (IAM) pathway, are also considered to contribute to IAA biosynthesis under specific conditions"