infB (PA4744, Q9HV55) review notes

Translation initiation factor IF-2, a TRAFAC translational GTPase (HAMAP MF_00100).

Literature (no deep-research provider available; own reading)

Steiner-Mosonyi et al. 2004 (abstract only; no PMC full text): P. aeruginosa
can initiate translation with both fMet-tRNAfMet and unformylated Met-tRNAfMet,
and its IF2 is required for the formylation-independent route PMID:15385567.
E. coli IF2 could not substitute; changing the charge of the aminoacyl-binding
cleft swapped the behaviour PMID:15385567. This is genetic and
structure-function evidence that PA IF2 acts directly in initiation, and
supports GO:0006413 (and, by the cleft mutagenesis, initiator tRNA
discrimination as part of the factor's own activity).
UniProt (HAMAP-Rule MF_00100): IF2 protects fMet-tRNA, promotes its binding to
the 30S subunit, and hydrolyses GTP during 70S complex formation.
PubMed MCP search returned API errors on 2026-11-05.

PseudoCAP IBA-seed assessment

Seed support queried live in QuickGO on 2026-11-05 (MOD ids mapped to UniProt).

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN001897651	GO:0006413 translational initiation	7	326 (141 taxa)	GO:0006413, IMP, PMID:15385567 (PseudoCAP)	SOUND	The abstract positively states that PA IF2 is required for (formylation-independent) initiation and maps the effect to the factor's tRNA-binding cleft; the process term is the factor's own process.	E. coli IF2 P0A705 (IDA PMID:4943554), yeast FUN12/eIF5B P39730 (IDA/IMP, GO:0001732), Candida FUN12 Q5A782 (IGI), S. pombe mti2 O59683 (EXP/IMP, GO:0070124), human MTIF2 P46199 (IDA GO:0001731), bovine MTIF2 P46198 (IDA GO:0070124)	Yes; several IDA/EXP seeds in bacteria, mitochondria and eukaryotic eIF5B

The node spans bacterial IF2, mitochondrial IF2 and eukaryotic eIF5B; the generic
GO:0006413 suits a clade of that breadth. No PSEUDOCAP or GO_CENTRAL defect.

Cytoplasm IBA at the same node: many seeds are mitochondrial (still part_of
cytoplasm), so the node term is fine; for PA4744 the specific cytosol IBA
(PTN000562019, from E. coli IF2) is accepted, and the cytoplasm rows are
TOO_GENERAL (cytosol part_of cytoplasm).