Q8IPI4 is the 424-residue Mst27D product. PMID:37428798(https://pubmed.ncbi.nlm.nih.gov/37428798/), DOI https://doi.org/10.1371/journal.pgen.1010837, uses Mst27D-PA and explicitly defines the C-terminal region as residues 151–424, consistent with the selected full-length model. Full text was inspected. It directly distinguishes intact Mst27D lattice binding from EB1 plus-end tracking, and native postmeiotic expression from premature expression caused by SV40-terminated transgenes. These are biological grounds to challenge specific EB1-derived IBA transfers rather than donor-count arguments. Nine original GOA rows are retained with one new bundling annotation. ProtNLM microtubule binding is CNN; a questionable finer GOA annotation is not used to force LSP.
The selected frozen UniProt record is retained in Mst27D-uniprot-source.json, with the complete original prediction metadata in Mst27D-predictions-source.json. It maps the exact accession to this FlyBase gene; current sequence identity does not establish the historical predictor input. Gene-level experiments support conserved functions unless a relevant isoform difference is established. Falcon research was requested with perplexity-lite fallback alongside publication caching. Primary publications and sequence observations, rather than AI summaries or ARBA assertions, support the curated conclusions.
The wrapper timed out and its fallback reported insufficient quota, but the original Falcon job later produced a complete report. The report and primary sources were inspected; no duplicate provider submission was made. The report supports Nup358 linkage and lattice-wide binding. Its mention of meiotic spindle enrichment must be interpreted with the primary study’s explicit correction for premature expression from SV40-terminated constructs. The endogenous-terminator and antibody experiments establish native accumulation after meiosis.
Re-read all 10 annotation rows and the existing Falcon report against the full primary study PMID:37428798. Fetched and inspected PTHR10623 PAINT: the eight original IBA rows trace to eukaryotic PTN000065701. The primary paper supports native postmeiotic expression, direct microtubule binding/bundling and Nup358 recruitment. The existing NEW bundling term is retained on biochemical/cellular assembly evidence plus stage-resolved mutant/rescue data, not solely on a fertility phenotype.
Plus-end binding/location remain UNDECIDED pending focused adjudication: "Mst27D binds all along MTs, contrasting with Eb1’s preference for growing MT plus ends" demonstrates altered preference, whereas GO:0051010 requires binding and does not specify preference. The comet-forming construct was the isolated C-terminal region, not a protein lacking the C terminus; the authors discuss possible heterodimerization with endogenous EB-family proteins. MTOC localization is assessed separately from spindle midzone, because GO:0005815 includes nucleating/anchoring structures outside mitosis. Polymerization/depolymerization regulation remains unresolved because the demonstrated bundling mechanism does not by itself adjudicate dynamic regulation.
The spindle-assembly rejection remains supported by target-specific native timing ("endogenous Mst27D accumulation starts after meiosis in early spermatids") and null analysis ("Until after meiosis, abnormalities were not detectable."). Midzone remains cautiously over-annotated. This is a branch/context challenge, not an argument from sparse or distant donors.