Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis.
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High-throughput proteomics of the Arabidopsis chloroplast envelope membrane; the curated PDS hit in this dataset underlies the chloroplast-envelope HDA annotation.
"identify and characterize membrane proteins localized to the Arabidopsis thaliana chloroplastic envelope membrane"
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
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Large-scale MS analysis of Arabidopsis chloroplasts assigned ~916 nuclear-encoded proteins with high confidence to the plastid; the curated PDS hit underlies the chloroplast HDA annotation.
"916 nuclear-encoded proteins were assigned with high confidence to the plastid"
AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.
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AT_CHLORO assigns subplastidial (stroma/thylakoid/envelope) localizations by semiquantitative spectral counting; the curated PDS hits underlie the chloroplast-envelope and chloroplast-thylakoid HDA annotations.
"accurate subplastidial localization of previously known and newly identified proteins"
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
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Protein-correlation profiling of Arabidopsis leaf fractions; over 150 proteins were dual-localized between cytosolic and membrane-associated pools, the context for the cytosol HDA hit on this transit-peptide-bearing plastid enzyme.
"were clearly partitioned between cytosolic and membrane-associated pools"
Regulation of phytoene desaturase expression is independent of leaf pigment content in Arabidopsis thaliana.
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PDS is nuclear-encoded and post-translationally imported into the plastid, and carries out the two-step desaturation of phytoene to zeta-carotene as one of the earliest steps of the carotenoid pathway.
"The two-step desaturation of phytoene to zeta-carotene, carried out by the enzyme phytoene desaturase (PDS)"
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Carotenoid synthesis in higher plants occurs in the plastids, the basis of the TAS chloroplast localization.
"Synthesis of carotenoids in higher plants occurs in the plastids"
Two Arabidopsis thaliana carotene desaturases, phytoene desaturase and zeta-carotene desaturase, expressed in Escherichia coli, catalyze a poly-cis pathway to yield pro-lycopene.
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Recombinant Arabidopsis phytoene desaturase and zeta-carotene desaturase, reconstituted with GGPP synthase and phytoene synthase in E. coli, produce pro-lycopene (7,9,7',9'-tetra-cis-lycopene) as the main end product of the plant poly-cis pathway.
"We show that pro-lycopene (7,9,7',9'-tetra-cis)-lycopene is the main end product of the plant desaturation pathway in these cells."
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The PDS product is zeta-carotene, which in the dark accumulates; illumination photoisomerizes it so that ZDS can act.
"Whereas in the dark mainly zeta-carotene, the phytoene desaturase product, accumulates"
UniProt entry Q07356 (PDS_ARATH)
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PDS catalyses 2 plastoquinone + 15-cis-phytoene = 9,9',15-tri-cis-zeta-carotene + 2 plastoquinol (EC 1.3.5.5), converting phytoene into zeta-carotene via phytofluene.
"Converts phytoene into zeta-carotene via the intermediary of"
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PDS is a plastid enzyme localized to chloroplast and chromoplast, and is a peripheral membrane protein.
"SUBCELLULAR LOCATION: Plastid, chloroplast. Plastid, chromoplast"
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PDS functions in carotenoid/lycopene biosynthesis.
"PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis."