PMID:30293966

The oligomeric plasticity of Hsp20 of Sulfolobus acidocaldarius protects environment-induced protein aggregation and membrane destabilization.

Hsp20 exists as a ~24-mer at room temperature and forms higher oligomeric forms at higher temperature (50-70 degrees C) and lower pH (3.0-5.0).

"Our data suggested the existence of a ~24-mer of Hsp20 at room temperature (25 °C) and a higher oligomeric form at higher temperature (50 °C-70 °C) and lower pH (3.0-5.0)"
The dimer is the functional conformation in the presence of aggregating substrate proteins.

"we identified a dimeric form of protein as the functional conformation in the presence of aggregating substrate proteins"
Hsp20 protects against stress-induced protein aggregation.

"it plays a key role in the protection of stress-induced protein aggregation"
Hsp20 interacts with membrane lipids via hydrophobic interaction and stabilizes membranes.

"Hsp20 interacts with membrane lipids via a hydrophobic interaction and it lowers the propensity of in vitro phase transition of bacterial and archaeal lipids"
Hsp20 has been found in secretory vesicles despite being a non-secreted protein.

"Hsp20, despite being a non-secreted protein, has been reported to be present in secretory vesicles"

PMID:34637594

Archaeal Hsp14 drives substrate shuttling between small heat shock proteins and thermosome: insights into a novel substrate transfer pathway.

Hsp20 forms hetero-oligomers with Hsp14 at 50-70 degrees C.

"We observed hetero-oligomer formation only at higher temperatures (50 °C-70 °C)"
Hsp20-captured substrates can be transferred via Hsp14 to the thermosome for refolding.

"Hsp14 could transfer sHsp-captured substrate proteins to Hsp60"

PMID:37516156

Heat shock response in Sulfolobus acidocaldarius and first implications for cross-stress adaptation.

hsp20 plays crucial roles in the majority of stress conditions including heat, oxidative, and nutrient stress.

"The results demonstrated that the gene thβ encoding the β subunit of the thermosome, as well as hsp14 and hsp20, play crucial roles in the majority of stress conditions"

PMID:32562000

Defining heat shock response for the thermoacidophilic model crenarchaeon Sulfolobus acidocaldarius.

Dynamic increase in mRNA levels of heat shock proteins upon heat shock.

"a dynamic increase in mRNA levels of all relevant heat shock proteins"

PMID:15995215

The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota.