Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Combined Automated Annotation using Multiple IEA Methods
Structure of the cell-puncturing device of bacteriophage T4.
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Determines the 2.9 A crystal structure of the (gp5-gp27)3 cell-puncturing complex, showing that three gp27 monomers form a hollow cylinder that binds the N-terminal domain of gp5 and extends the DNA ejection channel at the center of the baseplate.
"The amino-terminal, antiparallel beta-barrel domain of gp5 is inserted into a cylinder formed by three gp27 monomers, which may serve as a channel for DNA ejection."
Control of bacteriophage T4 tail lysozyme activity during the infection process.
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Solves the crystal structure of the non-cleaved gp5(S351L) mutant in complex with gp27, demonstrating how gp5 maturation and inhibition operate within the (gp5-gp27)3 central hub complex.
"The crystal structure of the non-cleavage phenotype mutant of gp5, S351L, complexed with gene product 27, showed that the 18 residues in the vicinity of the potential cleavage site (disordered in the wild-type structure) had visible electron density."
Structure of the T4 baseplate and its function in triggering sheath contraction.
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Presents the atomic structure of the ~6-MDa T4 baseplate in pre- and post-attachment states, defining the role of the central gp27 hub and surrounding wedge proteins in triggering sheath contraction.
"Here we present the atomic structure of the approximately 6-megadalton bacteriophage T4 baseplate in its pre- and post-host attachment states and explain the events that lead to sheath contraction in atomic detail."
UniProtKB record for P17172 (27_BPT4)
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gp27 forms a heteromultimer with the gp5 trimer and gp5.4 monomer at the center of the baseplate, creating an extension of the tail tube involved in tail assembly and infection.
"Heteromultimer with gp5 trimer and gp5.4 monomer; this interaction forms the tail lysozyme complex that creates an extension of the tail tube (PubMed:12837775, PubMed:27193680). The T4 tail lysozyme complex is made up of three copies of the proteolytically processed gp5 and three copies of gp27."
Deep research summary for Bacteriophage T4 gene 27 (gp27)
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gp27 forms the central hub scaffold around which the six baseplate wedges assemble and positions the gp5/gp5.4 puncturing spike at the tail tip.
"gp27 provides the central hub scaffold around which the six baseplate wedges assemble, largely through key contacts with gp6."