Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Electronic Gene Ontology annotations created by ARBA machine learning models
Genetic and structural characterization of the human mitochondrial inner membrane translocase.
Identification and characterization of a human mitochondrial homologue of the bacterial co-chaperone GrpE.
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Original identification of GRPEL1 (HMGE). Demonstrated mitochondrial localization by subcellular fractionation and immunocytochemistry. Showed binding to DnaK, Hsc70, and Mt-Hsp70. Demonstrated inhibition of HSJ1b-enhanced Hsc70 ATPase activity. Modeled on the E. coli GrpE crystal structure with significant structural conservation.
"HMGE expressed in E. coli as a GST fusion protein co-purified with the E. coli Hsp70 protein DnaK in the absence of ATP. DnaK could be released from the GST-HMGE with a Mg-ATP wash. ...Subcellular fractionation and immunocytochemistry studies using antisera raized against HMGE show that it is a mitochondrial protein."
Regulation of mitochondrial protein import by the nucleotide exchange factors GrpEL1 and GrpEL2 in human cells.
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Demonstrates that human GRPEL1/GrpEL1 acts with GRPEL2 as a mitochondrial NEF system for mtHsp70/HSPA9, regulating mtHsp70 function, preprotein import, Fe-S cluster biogenesis, and mitochondrial protein quality control.
"We found that both GrpEL1 and GrpEL2 associate with mtHsp70 as a hetero-oligomeric subcomplex and regulate mtHsp70 function. The formation of this subcomplex was critical for conferring stability to the NEFs, helped fine-tune mitochondrial protein quality control, and regulated crucial mtHsp70 functions, such as import of preproteins and biogenesis of Fe-S clusters."
Architecture of the human interactome defines protein communities and disease networks.
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
A reference map of the human binary protein interactome.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Falcon deep research for human GRPEL1