rrf-1 (G5ECM1) review notes

Provenance / process

• Fetched with just fetch-gene worm G5ECM1 --alias rrf-1. UniProt has three unreviewed rrf-1 entries (G5ECM1 1601 aa, Q7JLA0 1601 aa, G3MU40 1520 aa). G5ECM1 is the one cross-referenced to WormBase F26A3.8a and is the one used in modules/c_elegans_mutator_22g_rna_amplification.yaml.
• Deep research FAILED (falcon timeout; perplexity fallback not configured). No deep-research file was written. The review uses cached publications and PubMed.

Key findings

• Biochemical RdRP: PMID:18007599
• Mutator foci: PMID:22713602; purified mutator complexes make siRNAs in vitro PMID:24684932.
• pUG templates: PMID:32499657.
• Soma: PMID:21396820.

Curation decisions

• Protein binding (DRH-3) was modified to DEAD/H-box RNA helicase binding. Nucleotidyltransferase activity was modified to RNA-directed RNA polymerase activity.
• The nuclear RDRC (GO:0031380) IBA was marked as over-annotated. The RRF-1 complex acts in cytoplasmic Mutator foci, and among the RdRPs only EGO-1 is needed for meiotic H3K9me2 PMID:16271877.