Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened.
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LZTFL1 is a cytoplasmic protein (not enriched in cilia/basal body) that binds the BBSome via BBS9, self-associates into homo-oligomers, and negatively regulates BBSome and Smoothened ciliary trafficking; it acts specifically on the BBSome, not general IFT.
The centriolar satellite protein AZI1 interacts with BBS4 and regulates ciliary trafficking of the BBSome.
A proteome-scale map of the human interactome network.
Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Architecture of the human interactome defines protein communities and disease networks.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
LZTFL1 binds the BBSome and prevents its traffic to the cilium
Falcon deep research report for LZTFL1
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LZTFL1-specific findings: physical interaction with IFT27 (yeast two-hybrid, co-IP, colocalization, luciferase complementation; Huang 2021); direct binding to AP-1 (beta1) and AP-2 (beta2) via a conserved DxxFxxLxxxR motif with participation in TfR1 trafficking (Promchan 2020); and stage-specific testis localization to the developing flagellum and near the manchette during spermiogenesis (Huang 2021).