ampG (PA4393, G3XCT7) review notes

MFS permease of the AmpG/SLC33 family (TC 2.A.1.25.5; InterPro IPR004752
AmpG_permease/AT-1; PANTHER PTHR12778). Unreviewed UniProt entry. 594 aa,
14 TM helices by PhoA/LacZ fusions. Second gene of the ampF-ampG operon.

Literature (no deep-research provider available; own reading)

Zhang et al. 2010 (abstract only; PMC2976151 returned no full text via the
PubMed MCP on 2026-11-05). E. coli AmpG imports GlcNAc-anhMurNAc and its
peptides, the ampC inducer PMID:20713660.
PA4393 inactivation strongly lowers intrinsic beta-lactam resistance
PMID:20713660; cloned PA4393 complements P. aeruginosa and E. coli
ampG mutants PMID:20713660; PA4218 does not.
Kong et al. 2010 (full text read). AmpG topology: 14 TM helices
PMID:21192796; ampC promoter induction lost in ampG and ampP mutants
PMID:21192796. No transport assay.
IPR024371 (AcetylCoA_trans_1-like) InterPro2GO maps acetyl-CoA transport,
the function of the animal SLC33A1 branch; wrong for bacterial AmpG (rows
removed).

PseudoCAP IBA-seed assessment

Other seeds' experimental support queried live in QuickGO on 2026-11-05.

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN008542531	GO:0022857 transmembrane transporter activity	4	45 (40 taxa)	GO:0022857, IMP, PMID:20713660 (PseudoCAP)	SOUND	Abstract: PA4393 complements the E. coli ampG mutant, so it is the functional muropeptide permease. Generic term correct (gene-level refinement GO:0015647).	human SLC33A1 O00400 (IDA GO:0008521 acetyl-CoA transporter), E. coli AmpG P0AE16 (IDA GO:0015647), P. aeruginosa PA4218 Q9HWG8 (PseudoCAP IDA; evidence code defective, see PA4218 notes)	Yes; two independent IDA seeds

Node-level judgement (recorded here, the gene with the most direct
evidence, for the batch): the node spans bacterial AmpG-like permeases and
animal SLC33A1 acetyl-CoA transporters, so the generic transporter term is
the right level and the IBD is sound. No GO_CENTRAL defect. PA4218 shares the
node; its PseudoCAP IDA row has a WRONG_EVIDENCE_CODE defect (topology only)
but the IBD does not depend on it.

Other PseudoCAP rows (not seeds)

term	verdict	defect
GO:0009276 Gram-negative-bacterium-type cell wall (IMP)	WRONG	PSEUDOCAP WRONG_TERM, CONFIRMED: GO:0009276 is the peptidoglycan layer; AmpG is a 14-helix inner-membrane protein (topology, PMID:21192796 full text). Repoint to GO:0005886. Same defect on PA4218.
GO:0033252 regulation of beta-lactamase activity (IMP)	WRONG	PSEUDOCAP WRONG_TERM, PROBABLE (abstract only): AmpG imports the inducer of ampC expression; it does not act on the enzyme.
GO:0046677 response to antibiotic (IMP)	SOUND, non-core	none