Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
Connecting quorum sensing, c-di-GMP, pel polysaccharide, and biofilm formation in Pseudomonas aeruginosa through tyrosine phosphatase TpbA (PA3885).
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tpbB loss suppresses the tpbA mutant phenotypes; TpbA acts through TpbB.
"Loss of an uncharacterized GGDEF protein, PA1120 (TpbB), suppressed these phenotypes, indicating that TpbA controls c-di-GMP production through TpbB."
YfiBNR mediates cyclic di-GMP dependent small colony variant formation and persistence in Pseudomonas aeruginosa.
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Purified truncated YfiN synthesises c-di-GMP from GTP.
"In accordance with these findings, truncated-YfiN-His6 generated c-di-GMP from GTP, confirming that YfiN functions as a DGC (Figure 2A)."
Investigating the allosteric regulation of YfiN from Pseudomonas aeruginosa: clues from the structure of the catalytic domain.
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The HAMP-GGDEF construct is active in vitro and not product-inhibited.
"Here we show that, unlike other DGC enzymes, YfiN does not undergo product feedback inhibition, and that the in vitro activity depends on the presence of the HAMP domain."