NDE2 (YDL085W, UniProt Q07500) notes

Module: oxphos (external NDH-2, minor paralog). Absent from YeastCyc PWY3O-188 gene list.

Evidence journal

• nde2 single mutant has no effect on mitochondrial NADH oxidation; contribution only seen in nde1 nde2 PMID:9733747.
• PMID:9696750
• Mitochondrial supercomplex member PMID:11502169; IMS location from proteomics [UniProt:Q07500].
• No purified-enzyme data; MF activity annotations are ISS/IEA, supported indirectly by double-mutant phenotype.

Curation decisions

• Core MF GO:0120555 (by family + genetics), BP GO:0006120 (not in GOA; not added as NEW because NDE2-specific evidence is only the double mutant), CC inner membrane.
• pyruvate fermentation to ethanol: MARK_AS_OVER_ANNOTATED (indirect).