Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Interactions among APETALA1, LEAFY, and TERMINAL FLOWER1 specify meristem fate.
-
AP1 and LFY cooperatively assign floral fate to lateral meristems and antagonize TFL1 expression during the meristem fate transition.
"Assignment of floral fate to lateral meristems is primarily due to the cooperative activity of the flower meristem identity genes LEAFY (LFY), APETALA1 (AP1), and CAULIFLOWER."
-
AP1 can positively regulate LFY and repress TFL1-associated shoot meristem identity.
"We have found that AP1, in turn, can positively regulate LFY, because LFY is expressed prematurely in the converted floral meristems of plants constitutively expressing AP1."
Prenylation of the floral transcription factor APETALA1 modulates its function.
-
AP1 is described as a MADS-box transcription factor regulating the transition to floral meristems and sepal/petal development.
"The Arabidopsis MADS box transcription factor APETALA1 (AP1) was identified as a substrate for farnesyltransferase and shown to be farnesylated efficiently both in vitro and in vivo."
-
The cached abstract supports AP1-GFP functional assays but does not contain the AP1 nuclear localization details used by the original GOA IDA annotation.
"AP1 fused to green fluorescent protein (GFP) retained transcription factor activity and directed the expected terminal flower phenotype when ectopically expressed in transgenic Arabidopsis."
Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
-
Arabidopsis encodes a large transcription factor repertoire and many conserved DNA-binding domains.
"Arabidopsis dedicates over 5% of its genome to code for more than 1500 transcription factors, about 45% of which are from families specific to plants."
Complexes of MADS-box proteins are sufficient to convert leaves into floral organs.
-
AP1 participates with AP3/PI and SEP3 in higher-order MADS complexes sufficient to impose floral organ identity on leaves.
"Here we show that the class B proteins of Arabidopsis, PISTILLATA (PI) and APETALA3 (AP3), interact with APETALA1 (AP1, a class A protein) and SEPALLATA3 (SEP3, previously AGL9), and with AGAMOUS (AG, a class C protein) through SEP3."
-
AP1-containing MADS complexes can drive petaloid organ identity in ectopic expression assays.
"vegetative leaves of triply transgenic plants, 35S::PI;35S::AP3;35S::AP1 or 35S::PI;35S::AP3;35S::SEP3, are transformed into petaloid organs"
APETALA1 and SEPALLATA3 interact to promote flower development.
-
AP1 and CAL have overlapping flower meristem identity roles, and AP1 is later required for sepal and petal development.
"In Arabidopsis, the closely related APETALA1 (AP1) and CAULIFLOWER (CAL) MADS-box genes share overlapping roles in promoting flower meristem identity. Later in flower development, the AP1 gene is required for normal development of sepals and petals."
-
SEP3 interacts functionally with AP1 during normal flower development.
"Taken together, these studies suggest that SEP3 interacts with AP1 to promote normal flower development."
Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: new openings to the MADS world.
-
MADS-box transcription factors are plant developmental regulators and AP1 belongs to this transcription factor family.
"MADS-box transcription factors are key regulators of several plant development processes."
Defining subdomains of the K domain important for protein-protein interactions of plant MADS proteins.
-
The MADS domain mediates DNA binding and dimerization, while the K domain mediates protein-protein interactions in floral MADS proteins.
"All of these floral MADS proteins conserve two domains: the MADS domain that mediates DNA binding and dimerization, and the K domain that mediates protein protein interaction."
-
The cached abstract focuses on AP3/PI/SEP interactions and does not provide AP1-specific interaction evidence for retaining a generic protein binding annotation.
"Here we report on our studies on the interactions of the B class MADS proteins AP3 and PI with the E class MADS proteins SEP1, SEP2, and SEP3."
Comprehensive interaction map of the Arabidopsis MADS Box transcription factors.
-
A matrix yeast two-hybrid screen of Arabidopsis MADS-box proteins identified specific heterodimers and some homodimers.
"A matrix-based yeast two-hybrid screen of >100 members of this family revealed a collection of specific heterodimers and a few homodimers."
-
MADS protein interaction patterns help define floral induction and organ-formation networks.
"a model is proposed that integrates the floral induction and floral organ formation networks based on the interactions between the proteins involved."
Mutant analysis, protein-protein interactions and subcellular localization of the Arabidopsis B sister (ABS) protein.
-
The cached abstract supports ABS/TT16 interaction with floral homeotic proteins but does not provide AP1-specific text for the GOA AP1 protein binding row.
"Transgenic plants that ectopically express ABS show changes in the growth and identity of floral organs, suggesting that ABS can interact with floral homeotic proteins."
AGL24, SHORT VEGETATIVE PHASE, and APETALA1 redundantly control AGAMOUS during early stages of flower development in Arabidopsis.
-
AP1 and LFY downregulate AGL24 to establish floral meristem identity.
"AGL24 promotes inflorescence identity, and its expression is downregulated by APETALA1 (AP1) and LEAFY to establish floral meristem identity."
-
AP1-AGL24 and AP1-SVP dimers interact with the LUG-SEU corepressor complex.
"Protein interaction studies showed that dimers composed of AP1-AGL24 and AP1-SVP interact with the LUG-SEU corepressor complex."
APETALA1 and SEPALLATA3 interact with SEUSS to mediate transcription repression during flower development.
-
AP1 and SEP3 physically interact with SEU and can mediate transcriptional repression with SEU/LUG.
"Using the yeast two-hybrid assay and a co-immunoprecipitation assay, we showed that APETALA1 (AP1) and SEPALLATA3 (SEP3), both MADS box DNA-binding proteins, interacted with SEU."
-
AP1 and SEP3 may tether SEU/LUG corepressors to DNA regulatory elements.
"These results suggest that AP1 and SEP3 may serve as the DNA-binding partners of SEU/LUG."
Specification of Arabidopsis floral meristem identity by repression of flowering time genes.
-
AP1 specifies floral meristem identity in part by directly repressing SVP, AGL24, and SOC1 in emerging floral meristems.
"We show here that the emerging floral meristems require AP1 to partly specify their floral identities by directly repressing a group of flowering time genes, including SHORT VEGETATIVE PHASE (SVP), AGAMOUS-LIKE 24 (AGL24) and SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1)."
-
AP1 binds the cis-regulatory regions of these target genes in vivo.
"By post-translational activation of an AP1-GR fusion protein and chromatin immunoprecipitation assays, we further demonstrate the repression of these flowering time genes by induced AP1 activity and in vivo AP1 binding to the cis-regulatory regions of these genes."
Arabidopsis BLADE-ON-PETIOLE1 and 2 promote floral meristem fate and determinacy in a previously undefined pathway targeting APETALA1 and AGAMOUS-LIKE24.
-
LFY and AP1 expression in inflorescence-meristem primordia confers floral fate.
"In Arabidopsis, LEAFY (LFY) and APETALA1 (AP1) are key regulators of this transition and expression of these genes in primordia produced by the inflorescence meristem confers floral fate."
-
bop1 bop2 ap1 triple mutants convert flowers into highly branched inflorescence-like shoots, supporting AP1 involvement in floral meristem determinacy.
"The most dramatic changes occur in bop1 bop2 ap1-1 triple mutants where flowers are converted into highly branched inflorescence-like shoots."
Evidence for network evolution in an Arabidopsis interactome map.
-
The paper reports a proteome-wide binary interaction map; AP1 rows from this source should not be treated as AP1 core function without independent biological validation.
"We describe a proteome-wide binary protein-protein interaction map for the interactome network of the plant Arabidopsis thaliana containing about 6200 highly reliable interactions between about 2700 proteins."
Characterization of MADS-domain transcription factor complexes in Arabidopsis flower development.
-
AP1, AP3, PI, AG, and SEP3 interact in floral tissues in a manner consistent with the floral quartet model.
"Here, we show using affinity purification and mass spectrometry that five major floral homeotic MADS-domain proteins (AP1, AP3, PI, AG, and SEP3) interact in floral tissues as proposed in the "floral quartet" model."
-
MADS-domain protein complexes can coexist in the nucleus and bind CArG boxes in target promoters.
"Based on our in vitro EMSA studies, we propose that different heteromeric MADS-domain protein complexes can coexist within the nucleus and may compete for partly overlapping sets of DNA-binding sites."
Phytoplasma effector SAP54 hijacks plant reproduction by degrading MADS-box proteins and promotes insect colonization in a RAD23-dependent manner.
-
Phytoplasma effector SAP54 interacts with AP1 and other MADS-domain transcription factors and promotes their degradation.
"We find that phytoplasma produce a novel effector protein (SAP54) that interacts with members of the MADS-domain transcription factor (MTF) family, including key regulators SEPALLATA3 and APETALA1, that occupy central positions in the regulation of floral development."
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping.
-
CrY2H-seq produced a high-throughput Arabidopsis transcription factor interactome; AP1 interactions from this source are useful as screening data but are not sufficient to make generic protein binding a core AP1 function.
"We applied CrY2H-seq to comprehensively screen a collection of 1,956 Arabidopsis transcription factors and regulators (hereafter collectively called TFs)"
-
The study reports thousands of binary TF interactions.
"We report a deep coverage Arabidopsis transcription factor interactome composed of 8,577 binary interactions, 7,994 of which are novel."
A PXY-Mediated Transcriptional Network Integrates Signaling Mechanisms to Control Vascular Development in Arabidopsis.
-
The paper maps a high-throughput transcription factor-promoter network for vascular development; the cached text does not discuss AP1 specifically.
"Here, we mapped a putative PXY-mediated transcriptional regulatory network comprising 690 transcription factor-promoter interactions in Arabidopsis (Arabidopsis thaliana)."
Protein interaction mapping reveals widespread targeting of development-related host transcription factors by phytoplasma effectors.
-
The paper reports a broad phytoplasma effector-host transcription factor interaction network and should be treated as host-pathogen screening context rather than AP1 core function evidence.
"we generated a protein-protein interaction network between a broad set of phytoplasma effectors and a large, unbiased collection of Arabidopsis thaliana transcription factors and transcriptional regulators."
UniProt record P35631 for APETALA1
-
UniProt describes AP1 as a nuclear MADS-box transcription factor that promotes early floral meristem identity, controls sepal/petal development, interacts with MADS partners, and represses flowering-time genes in emerging floral meristems.
Falcon deep research report for Arabidopsis AP1
-
The Falcon report synthesizes AP1 as a MIKC-type MADS transcription factor, floral meristem identity regulator, context-dependent activator/repressor, and member of higher-order floral MADS complexes; it also highlights recent AP1 chromatin/pioneer-factor literature that is useful context but not yet present in GOA.
PANTHER family metadata for PTHR48019
-
PTHR48019 is the broad MADS-box/MEF2 transcription factor family; its description supports conserved DNA binding, transcriptional regulation, dimerization, and flower-development roles in plants, but the family is too broad for AP1-specific floral identity transfer without direct evidence.