The review uses HGNC:2934 / UniProt Q9Y5R6 and preserves all 38 normal-fetch annotation source records, including their qualifiers and supporting entities. The historic alias DMT1 must not be confused with the unrelated metal transporter. Three official predicted products were reconstructed and independently matched to official FASTAs. No prior main review or history existed at the fixed intake boundary.
The 38 decisions comprise 20 ACCEPT, 9 MODIFY, 8 KEEP_AS_NON_CORE and 1 UNDECIDED. No NEW annotation is added. One nuclear transcription-factor core integrates activation and repression, male differentiation and the male germ-cell meiotic-entry gate. Short verbatim anchors occur once in the YAML core; repeated evidence support is reference-only. Detailed public source locators and access limits are in the evidence guide.
Human DNA recognition, full-length protein crosslinking and testis chromatin occupancy in PMID:26005864(https://pmc.ncbi.nlm.nih.gov/articles/PMC4476070/) support the integrated mechanism. Full-length experiments are distinguished from the 67–136 structural fragment; mouse ChIP-exo is distinguished from human ChIP-seq. The earlier human DMRT1–VP16 reporter in PMID:17605809(https://pubmed.ncbi.nlm.nih.gov/17605809/) is not sufficient alone to establish native activation. Independent inducible human DMRT1 HEK293 DAZL cis-regulatory reporter experiments in PMID:37709822(https://pubmed.ncbi.nlm.nih.gov/37709822/) and mouse Sohlh1 regulation in PMID:20951351(https://pmc.ncbi.nlm.nih.gov/articles/PMC2996490/) provide that support.
Developmental claims retain their exact contexts. Mouse fetal ovarian cytoplasmic staining in PMID:21621532(https://pubmed.ncbi.nlm.nih.gov/21621532/) is positive evidence for a transient pool, not an inference from the absence of a signal peptide. Mouse spermatogonial proliferation and induced human germ-cell mitotic arrest refer to different stages. High transgene expression and timing control may also accelerate the latter program. The DAZL experiments demonstrate activation of genomic-peak reporters, not endogenous human physiology or exclusively promoter activity. Motif-deletion constructs were tested, but no directional deletion effect is claimed without reading the figure/source data. The specific male-fate specification annotation is retained contextually; the summary does not call DMRT1 a mammalian SRY-like initiating switch. Human fetal-tissue depletion in PMID:30272154(https://pubmed.ncbi.nlm.nih.gov/30272154/) supports later testicular differentiation but does not prove first-trimester dispensability after partial depletion.
The sole unresolved annotation is the rat-derived intracellular signal-transduction claim. Exact donor Q925A6 carries IDA PMID:11181532. Available text establishes upstream regulation of Dmrt1 expression, while full-original access remains blocked; a performing signal-relay step could not be adjudicated. No experimental removal or wrong-gene allegation is made.
All five PAINT functions were traced to the actual ancestral node and target path. The negative branch in the same family does not contain human DMRT1. Target self-grounding for nucleus is legitimate. ARBA and InterPro mappings were checked against actual target features and current rule contents. The more specific replacements preserve the broad source identities and are not new annotations. The evidence-code difference between duplicate claims does not change their biological verdict.
Product uncertainty is retained explicitly: DMRT1c preserves the annotated feature through residue 118 but changes the segment containing the experimentally important R123. Neither equivalent activity nor inactivity follows from those sequence facts. DMRT1b retains the DNA-recognition region but loses a substantial C-terminal segment. The transcript paper PMID:16617334(https://pubmed.ncbi.nlm.nih.gov/16617334/) is abstract-only and is not treated as a biochemical comparison.
Machine inputs and publication bytes were never manually rewritten. The PMID:21775990 cache remains abstract-only despite independently recovered and read original PMC text; its erratum corrects figure labels. The PMID:17540358 cache has a positive full-text flag but lacks complete Results/Methods in its body. The attempted PMID:20616082 normal fetch remains a recorded failure. Falcon authentication and genuine fallback quota failures produced no provider report; none was invented. Original access failures and all consulted source boundaries remain documented.
The headline now identifies DMRT1 as a sequence-specific transcription factor. This narrower wording does not reject its structurally demonstrated zinc coordination: the original human 67–136 fragment crystallography reports PMID:26005864(https://pmc.ncbi.nlm.nih.gov/articles/PMC4476070/), in Online Methods, X-ray crystallography. The Results describe the zinc-containing recognition module at the DNA interface. No separate NEW metal-binding annotation is added merely to make the annotation list repeat an adjective from the description.
Short cache-exact anchors have been added once for the principal regulatory and localization claims. The mouse Stra8 repression and meiotic-entry gate, and Sohlh1 promoter occupancy, are anchored in the corresponding 2010 study, Results and Discussion. Reference-only support reuses these observations on duplicate source rows without repeating quotations. The core description now distinguishes direct transcriptional gating of Stra8 from downstream proliferation outcomes; all existing core terms and row verdicts remain unchanged.
The human DAZL reporter response is anchored to the Results accompanying Figure 6i in PMID:37709822(https://pubmed.ncbi.nlm.nih.gov/37709822/). Its induced-expression limitation is also explicit: the authors caution that high transgene levels PMID:37709822(https://pubmed.ncbi.nlm.nih.gov/37709822/), Discussion. The quotation does not establish endogenous timing or a directional effect of the motif deletions. The positive fetal-ovary location anchor is from PMID:21621532(https://pubmed.ncbi.nlm.nih.gov/21621532/), Results and Figure 1D–F; the fetal-testis differentiation anchor is from PMID:30272154(https://pubmed.ncbi.nlm.nih.gov/30272154/), Discussion/conclusion, with second-trimester tissue and partial-depletion limits retained in the reason.
This appendix supersedes the earlier statement that all short source anchors occur only in the core. Each excerpt occurs once across the authored YAML, notes and guide; combined quotation totals remain at most 25 words per source, including the two inherited core excerpts. The guide identifies the corresponding full-source locations. All 38 imported annotation objects, 38 actions, three predicted products, source-cache bytes and earlier notes are preserved. No new scientific assertion is introduced through the added quotations.