Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Two C. elegans histone methyltransferases repress lin-3 EGF transcription to inhibit vulval development.
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MET-2 is homologous to human SETDB1, an H3K9 HMT that represses transcription
"met-2 is homologous to human SETDB1, an H3K9 HMT that represses transcription"
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met-1 and met-2 are each required for normal trimethylation of both H3K9 and H3K36
"met-1 and met-2 (1) are each required for the normal trimethylation of both H3K9 and H3K36"
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met-1 and met-2 act redundantly with C. elegans HP1 homologs
"act redundantly with each other as well as with the C. elegans HP1 homologs"
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MET-2 represses transcription of lin-3 EGF gene
"repress transcription of the EGF gene lin-3, which encodes the signal that induces vulval development"
Differential localization and independent acquisition of the H3K9me2 and H3K9me3 chromatin modifications in the Caenorhabditis elegans adult germ line.
H3K9me2/3 binding of the MBT domain protein LIN-61 is essential for Caenorhabditis elegans vulva development.
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LIN-61, HPL-2, and MET-2 function together in vulva development
"establish interplay of the H3K9me2/3 binding proteins, LIN-61 and HPL-2, as well as the H3K9MT MET-2 in distinct developmental pathways"
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H3K9me2/3 interaction is central to vulval cell fate determination
"Interestingly, lin-61 genetically interacts with two other synMuvB genes, hpl-2, an HP1 homologous H3K9me2/3 binding factor, and met-2, a SETDB1 homologous H3K9 methyl transferase (H3K9MT), in determining C"
Caenorhabditis elegans histone methyltransferase MET-2 shields the male X chromosome from checkpoint machinery and mediates meiotic sex chromosome inactivation.
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MET-2 mediates meiotic sex chromosome inactivation (MSCI)
"that MET-2 also mediates the transcriptional silencing program of meiotic sex chromosome inactivation"
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MET-2 blocks checkpoint signaling on the partnerless male X chromosome
"Absence of MET-2 triggers the recombination checkpoint in worms with a single X"
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Loss of met-2 causes increased apoptosis through checkpoint activation
"Absence of met-2 in X0 germ lines results in increased apoptosis by activating the recombination checkpoint"
Step-wise methylation of histone H3K9 positions heterochromatin at the nuclear periphery.
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MET-2 mediates H3K9 mono- and dimethylation
"The two HMTs target H3K9 in a consecutive fashion: MET-2, a SETDB1 homolog, mediates mono- and dimethylation"
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SET-25 mediates H3K9 trimethylation
"SET-25, a previously uncharacterized HMT, deposits H3K9me3"
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MET-2 and SET-25 together position heterochromatin at nuclear periphery
"elimination of two HMTs, MET-2 and SET-25, mimics the loss of SAM synthetase, abrogating the perinuclear attachment of heterochromatic transgenes"
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MET-2 is a SETDB1 homolog
"MET-2, a SETDB1 homolog, mediates mono- and dimethylation"
Histone methylation restrains the expression of subtype-specific genes during terminal neuronal differentiation in Caenorhabditis elegans.
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MET-2 silences subtype-specific genes during neuronal differentiation
"MET-2 is the C. elegans homolog of human SETDB1 and Drosophila Eggless"
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met-2 mutants show ectopic unc-4 expression in VC neurons
"Endogenous unc-4 transcripts accumulate in all six VC neurons in pqe-1, cec-3, and met-2 mutants"
A histone methylation network regulates transgenerational epigenetic memory in C. elegans.
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MET-2 required for small-RNA-induced H3K9 methylation
"identified multiple chromatin-modifying factors, including H3K4me1/me2 and H3K9me3 methyltransferases"
SPR-5 and MET-2 function cooperatively to reestablish an epigenetic ground state during passage through the germ line.
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MET-2 functions with SPR-5 in germline epigenetic reprogramming
"SPR-5 and MET-2 function cooperatively to reestablish an epigenetic ground state"
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spr-5;met-2 double mutants have synergistic sterility
"spr-5;met-2 double mutants have a synergistic effect on sterility, H3K4me2, and spermatogenesis expression"
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MET-2 contributes to transgenerational epigenetic inheritance
"mutants in the H3K9me2 methyltransferase, met-2, result in transgenerational epigenetic effects"
The Nrde pathway mediates small-RNA-directed histone H3 lysine 27 trimethylation in Caenorhabditis elegans.
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MET-2 required for small-RNA-induced H3K9 methylation
"Whereas set-25 and met-2 are required for K9 methylation, mes-2 is required for K27 methylation"
Histone H3K9 methylation is dispensable for Caenorhabditis elegans development but suppresses RNA:DNA hybrid-associated repeat instability.
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MET-2 and SET-25 together protect repeat-rich genomic regions
"In met-2 set-25 double mutants, which lack all H3K9 methylation (H3K9me), embryos differentiate normally"
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H3K9 methylation suppresses transcription-induced replication stress
"H3K9me2 or H3K9me3 stabilizes and protects repeat-rich genomes by suppressing transcription-induced replication stress"
Deep research summary for MET-2
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MET-2 nuclear accumulation times heterochromatin onset
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LIN-65 rate-limits MET-2 nuclear entry
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ARLE-14 stabilizes MET-2 chromatin association
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Approximately 10-fold H3K9me2 increase at gastrulation
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MET-2 forms nuclear foci with LIN-65
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met-2 mutants abolish perinuclear anchoring of autosomal arms
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H3K9me2 (not me3) is required for lamina tethering
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Catalytically inactive MET-2 still forms foci and maintains some gene silencing
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Noncatalytic function involves constraining histone acetylation