Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
New early zygotic regulators expressed in endomesoderm of sea urchin embryos discovered by differential array hybridization.
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Spgcm was discovered as a beta-catenin-dependent zygotic regulator expressed exclusively in part of the prospective secondary mesenchyme at late cleavage.
"Spgcm, an orthologue of the fruit fly gene glial cells missing, which is first expressed specifically and exclusively in part of the prospective secondary mesenchyme (mesodermal) domain at late-cleavage blastula stage"
cis-regulatory processing of Notch signaling input to the sea urchin glial cells missing gene during mesoderm specification.
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gcm morpholino knockdown gives larvae without pigment cells.
"Here, we show that microinjection of a spgcm antisense morpholino oligonucleotide results in larvae without pigment cells."
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gcm is a direct target of Su(H)-mediated Notch signalling through conserved Su(H) sites in its middle cis-regulatory module.
"confirming that spgcm is a direct target of canonical N signaling mediated through Su(H) inputs"
Cis-regulatory logic driving glial cells missing: self-sustaining circuitry in later embryogenesis.
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The gcm late cis-regulatory module depends on Gcm and Six1 sites and forms a self-sustaining feedback loop that locks down pigment cell fate.
"Cis-perturbation analyses reveal that the two critical elements within this late module are consensus matches to Gcm and Six1 binding sites."
Cis-regulatory analysis of the sea urchin pigment cell gene polyketide synthase.
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Gcm is a direct positive regulator of pks through binding sites in the -1.5 kb cis-regulatory region.
"The mutagenesis of these DNA-binding sites indicated that SpGcm, SpGataE and SpKrl are direct positive regulators of SpPks."
Diversification of oral and aboral mesodermal regulatory states in pregastrular sea urchin embryos.
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gataE is a direct Gcm target; Gcm MASO abolishes the pigment differentiation battery.
"As expected, Gcm MASO causes severe decreases in transcript levels of the differentiation genes bpnt, dopt, fmo, papss, pks, and sult."
A comprehensive analysis of Delta signaling in pre-gastrular sea urchin embryos.
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gcm is the first gene activated by Delta/Notch signalling in the NSM and Gcm is a feed-forward activator of gataE.
"gcm was previously shown to be a direct cis-regulatory target of the Nic/Su(H) complex (Ransick and Davidson, 2006), and is the first gene to become activated by D/N signaling."
Synthetic in vivo validation of gene network circuitry.
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Forced gcm expression in skeletogenic cells diverts them to pigment cell formation, showing gcm is sufficient for the pigment cell program.
"The result of reengineering the regulatory circuitry in this way was to divert the developmental program of these cells from skeletogenesis to pigment cell formation, confirming a direct prediction of the GRN."
Global regulatory logic for specification of an embryonic cell lineage.
Repression of mesodermal fate by foxa, a key endoderm regulator of the sea urchin embryo.
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FoxA represses gcm in the veg2 endoderm, excluding mesodermal fate.
"a crucial role of Foxa is to repress gcm expression in response to a Notch signal, and hence to repress mesodermal fate"
Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening.
Roles of hesC and gcm in echinoid larval mesenchyme cell development.
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gcm knockdown in two other euechinoids confirms its role in pigment cell differentiation and reveals skeletogenic fate repression.
"We confirmed previous results by demonstrating that gcm is involved in pigment cell differentiation."