Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate
Original wording and all model/source metadata are retained in awd-protnlm-source.json.
CNN: supported, already represented by the NDP-kinase annotation. The exact target contains the complete 153-residue experimentally characterized awd enzyme with a 15-residue N-terminal extension. Catalytic and binding residues are preserved, supporting the phosphate-transfer mechanism described. Primary purification and mutagenesis studies establish fly awd activity; sequence analysis connects those results to the selected product.
PMID:1320004(https://pubmed.ncbi.nlm.nih.gov/1320004/) describes purified native Drosophila NDP kinase; PMID:7559441(https://pubmed.ncbi.nlm.nih.gov/7559441/) tests catalytic-site variants. The reviewed same-gene P08879 donor record supplies the established phosphohistidine mechanism. Current FlyBase and UniProt disagree about the N-terminal initiation, documented in the analysis; this does not invalidate the preserved catalytic core.