glp-1 (C. elegans) curation notes

UniProt P13508, "Protein glp-1" (contains glp-1/Notch intracellular domain). Second C. elegans Notch receptor
(paralog of lin-12). PANTHER (UniProt DR lines, verbatim): PTHR45836 (SLIT HOMOLOG), PTHR45836:SF23
(NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1). No IBA/PAINT rows in the GOA file for glp-1.

Receptor

• Receiving cell for the distal tip cell (DTC) signal: PMID:3677168
• Integral membrane protein in the distal mitotic zone: PMID:7607080
• Ligands LAG-2 (DTC) and APX-1 [PMID:7607081; PMID:19502484 redundant LAG-2/APX-1 in DTC signaling].
• Domain architecture: 10 EGF-like, 3 LNG (LNR), 6 ankyrin repeats PMID:1457827.

Nuclear function

• RAM and ANK bind LAG-1; ANK repeats are strong transcriptional activators in yeast PMID:9003776.
• Ternary complex with LAG-1 and LAG-3 PMID:10830967.
• Only two primary germline targets, lst-1 and sygl-1 PMID:32196486. These encode post-transcriptional
  regulators that act with FBF, so GLP-1's effects on FBF-2 protein are indirect (MARK_AS_OVER_ANNOTATED
  for post-transcriptional regulation of gene expression).

Variant biology (for the module)

• Maternal glp-1 mRNA is translationally regulated, so GLP-1 protein is restricted to anterior blastomeres
  PMID:9015263. APX-1 from P2 signals to ABp. This is a worm-specific use of Notch in early embryonic
  asymmetry.
• Germline stem cell niche signaling (DTC LAG-2 -> germline GLP-1) is a canonical but tissue-specific use of
  the pathway. GLP-1 and LIN-12 are largely interchangeable biochemically PMID:1769331.
• GLP-1 in dauer neurons maintains dauer PMID:18599512.

Deep research status

The first falcon run (just deep-research-falcon worm glp-1 --fallback perplexity-lite) timed out at 600 s. The
perplexity fallback is unavailable in this environment. A rerun with --timeout 2400 succeeded:
glp-1-deep-research-falcon.md. It is consistent with the review and is cited in core_functions. Note that
falcon reports C. elegans LIN-12/GLP-1 are tuned to lower force thresholds for activation than Drosophila
Notch (Langridge et al. 2021 bioRxiv; not in the publications cache, so not used as evidence here).