Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Gene Ontology annotation of human sequence-specific DNA binding transcription factors (DbTFs) based on the TFClass database
Combined Automated Annotation using Multiple IEA Methods
FOXC2 is a winged helix gene that counteracts obesity, hypertriglyceridemia, and diet-induced insulin resistance.
The murine winged helix transcription factors, Foxc1 and Foxc2, are both required for cardiovascular development and somitogenesis.
Analysis of the phenotypic abnormalities in lymphoedema-distichiasis syndrome in 74 patients with FOXC2 mutations or linkage to 16q24.
The establishment of a predictive mutational model of the forkhead domain through the analyses of FOXC2 missense mutations identified in patients with hereditary lymphedema with distichiasis.
Foxc2 is a common mediator of insulin and transforming growth factor beta signaling to regulate plasminogen activator inhibitor type I gene expression.
Transcription elongation regulator 1 is a co-integrator of the cell fate determination factor Dachshund homolog 1.
Identification of de novo mutations and rare variants in hypoplastic left heart syndrome.
A proteome-scale map of the human interactome network.
Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling.
Impact of cytosine methylation on DNA binding specificities of human transcription factors.
A reference map of the human binary protein interactome.
Clustered arrangement of winged helix genes fkh-6 and MFH-1: possible implications for mesoderm development.
Isolation of the mouse (MFH-1) and human (FKHL 14) mesenchyme fork head-1 genes reveals conservation of their gene and protein structures.
The Foxc2 transcription factor regulates angiogenesis via induction of integrin beta3 expression.
Forkhead transcription factors regulate expression of the chemokine receptor CXCR4 in endothelial cells and CXCL12-induced cell migration.
FOXC2 represses NFAT1-dependent transcription through a DNA-facilitated protein-protein interaction.
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Purified human FOXC2 residues 72–172 bind human NFAT1 residues 392–678 directly; ARRE DNA strengthens binding in ITC and SPR. Three ternary crystal structures and interface-mutant binding assays support the contact.
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Full-length FOXC2 represses NFAT1-driven IL2-ARRE2 reporter activity and target RNA abundance in transfected HEK293T cells. Interaction-interface mutations weaken repression; the NFAT element is required and the cognate FOX element is dispensable.
TRIM27 negatively regulates NOD2 by ubiquitination and proteasomal degradation.
Phosphorylation regulates FOXC2-mediated transcription in lymphatic endothelial cells.
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Endogenous human lymphatic endothelial FOXC2 shows phosphatase-sensitive mobility. Six serine sites were mapped by mass spectrometry and two additional sites by mutagenesis in overexpressed protein.
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Human lymphatic endothelial cells expressing Myc-tagged wild-type or eight-site mutant FOXC2 show different chromatin occupancy and transcriptional profiles. The mutant retains nuclear localization and binding to naked DNA in extract EMSA.
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Induced wild-type FOXC2, but not the phosphorylation-deficient mutant, increases vascular remodeling in embryonic mouse blood endothelium; the model did not target lymphatic endothelium.
FOXC2 controls formation and maturation of lymphatic collecting vessels through cooperation with NFATc1.
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Mouse Foxc2 deficiency prevents normal collecting lymphatic vessel maturation; calcineurin inhibition potentiates valve and remodeling defects in Foxc2 heterozygotes. These experiments retain their mouse and pharmacological scope.
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Endogenous FOXC2 ChIP-chip identifies 2,350 genomic sites in primary human intestinal lymphatic endothelial cells. Selected fragments bind FOXC2 in extract EMSA, and their reporter responses with NFATc1 in transfected 293T cells are context dependent.