GO_REF:0000041

Gene Ontology annotation based on UniPathway vocabulary mapping

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping

UniProt subcellular-location mapping supplies the cell outer membrane annotation.

file:PSEPK/algE/algE-uniprot.txt

UniProtKB reviewed entry for algE

UniProt describes AlgE as an outer-membrane AlgE-family protein with channel-forming properties and an alginate biosynthesis pathway role.

file:PSEPK/algE/algE-deep-research-falcon.md

Falcon deep research for algE

Falcon deep research for algE supports an outer-membrane alginate export porin function. The strongest experimental evidence is from characterized Pseudomonas AlgE homologs and an AlgEKX model including P. putida AlgE; no retrieved paper explicitly cites UniProt Q88NC8/PP_1284.

PMID:21778407

Structural basis for alginate secretion across the bacterial outer membrane.

Pa AlgE structure, liposome anion flux and native loop-mutant phenotypes support channel function; alginate translocation remains a mechanistic interpretation, not directly tracked polymer transport.

"monomeric 18-stranded β-barrel"

PMID:36494359

Structure of the AlgKX modification and secretion complex required for alginate production and biofilm attachment in Pseudomonas aeruginosa.

KT2440 Q88NC7 AlgK33–484 and AlgX1–479 provide direct structure/oligosaccharide binding. Native production/biofilm assays use Pa; AlgEKX is a predicted model, not an experimentally solved ternary complex.

"AlgKPp is capable of directly binding polyM and polyMG ligands"