Use of the ND evidence code for Gene Ontology (GO) terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Vam10p defines a Sec18p-independent step of priming that allows yeast vacuole tethering.
-
VAM10 deletion causes vacuole fragmentation in vivo, and recombinant Vam10p stimulates in-vitro fusion of purified vacuoles while anti-Vam10p antibody blocks it, establishing Vam10p as a positive factor required for homotypic vacuole fusion.
"VAM10 deletion causes vacuole fragmentation in vivo. The in vitro fusion of purified yeast vacuoles is stimulated by recombinant Vam10p and blocked by antibody to Vam10p."
-
Vam10p acts at an early, Sec18p-independent priming step and is required to restore Ypt7p-dependent tethering, placing it upstream of Rab-dependent tethering.
"Vam10p acts early in the priming stage of fusion, independent of Sec18p."