Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway.
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Recombinant Msed_1321, produced and purified anaerobically, is an active homotetrameric 4-hydroxybutyryl-CoA dehydratase.
"this suggests that the enzyme associates as a homotetramer"
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The M. sedula enzyme is much more oxygen tolerant than the clostridial homolog.
"With a half-life of roughly 4 days, 4-hydroxybutyryl-CoA dehydratase from M. sedula proved to be surprisingly robust in the presence of oxygen"
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Dehydration is slow and, with 4HB-CoA ligation, rate-limiting for the branch.
"these two reactions form the rate-limiting steps for the acetyl-CoA branch"
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Msed_1321 is strongly induced in autotrophic growth.
"4-hydroxybutyryl-CoA dehydratase (Msed_1321; 27-fold)"
Identification of missing genes and enzymes for autotrophic carbon fixation in crenarchaeota.
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Msed_1321 is the gene for the 4-hydroxybutyryl-CoA dehydratase activity of autotrophic Sulfolobales, while the paralog Msed_1220 lacks catalytic residues.
"one of which (Msed_1220) lacks catalytically essential amino acids"
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The dehydratase is the conserved key enzyme of the two crenarchaeal 4-hydroxybutyrate cycles.
"4-Hydroxybutyryl-CoA dehydratase, which catalyzes a mechanistically intriguing elimination of water, is well conserved and rightly can be considered the key enzyme of these two cycles."
Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic CO(2) Fixation in Metallosphaera sedula.
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The dehydratase is abundant and catalyses a slow radical reaction.
"Whereas 4-hydroxybutyryl-CoA dehydratase appears to constitute a large fraction of cellular protein as well, it slowly catalyzes a mechanistically difficult radical reaction"
A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea.
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Defines the 3HP/4HB cycle, including the 4-hydroxybutyryl-CoA dehydratase step.
"This intermediate was reduced to 4-hydroxybutyrate and converted into two acetyl-CoA molecules via 4-hydroxybutyryl-CoA dehydratase."