Falcon (Edison) deep research report for aldo-1 (C. elegans, P54216, T05D4.1)
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Falcon confirms P54216 identity as C. elegans fructose-bisphosphate
aldolase 1 (FBA1), citing a comparative aldolase phylogeny that lists the
UniProt accession directly.
"a comparative aldolase phylogeny explicitly lists “**Caenorhabditis elegans FBA 1 (P54216)**.”"
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Falcon describes ALDO-1 as a core glycolytic enzyme that cleaves
fructose-1,6-bisphosphate, with the gene aldo-1/T05D4.1 placed in glucose
metabolism toward glyceraldehyde-3-phosphate.
"catalyzes the cleavage of the substrate **fructose-1,6-bisphosphate (F1,6BP)** in glycolysis"
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Falcon notes the class I Schiff-base mechanism with a conserved active-site
lysine, drawing on a nematode aldolase analysis that includes C. elegans
aldo-1 in the sequence alignment.
"the lysine at position 230 is the residue where Schiff base intermediates are formed"
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Falcon reports kinetic parameters for a closely related nematode class-I
aldolase as supporting strong specificity/efficiency for F1,6BP (inference
for ALDO-1 from homology).
"**Km = 0.24 ± 0.01 µM** and **Vmax = 432 nmol·min⁻¹·mg⁻¹** at 30°C, with optimum pH **7.5**"
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Falcon reports that aldo-1 is transcriptionally upregulated in mitochondrial
complex I mutants (~3-fold in gas-1, ~6-fold in nuo-6), consistent with a
shift toward glycolysis.
"In *C. elegans* mitochondrial complex I mutants, aldo-1 is transcriptionally upregulated, consistent with a metabolic shift toward glycolysis"
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Falcon reports that aldo-1 RNAi knockdown strongly shortens lifespan of
complex I mutants, demonstrating functional importance for compensatory
glycolysis.
"decreased **gas-1(fc21)** median lifespan by **up to 65%**"
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Falcon reports the authors' interpretation that complex I mutants rely
heavily on glycolysis for energy, positioning ALDO-1 as a critical
component of compensatory energy metabolism.
"rely heavily on glycolysis for energy production"
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Falcon reports that persistent HIF-1 over-activation (vhl-1, egl-9, rhy-1
mutants) commonly upregulates aldo-1, annotated as fructose-1,6-bisphosphate
aldolase, within a metabolism-enriched gene set.
"is among the genes “commonly up-regulated” in all three mutants and is explicitly annotated as “**aldo-1 (fructose-1,6-bisphosphate aldolase)**.”"
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Falcon reports that in aging male neurons aldo-1 is among glycolysis genes
that decline with age, linking it to neuronal energetic aging.
"is listed among glycolysis enzymes that are “**downregulated with age at least 2-fold**” (Figure 5B) in male neurons"
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Falcon reports sex-biased expression with aldo-1/T05D4.1 higher in males
than hermaphrodites (male:hermaphrodite ratios ~2.1-2.85).
"shows higher expression in males than hermaphrodites"
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Falcon notes no direct C. elegans subcellular localization was retrieved in
its run, but family-level expectation places aldolase in cytosolic
glycolysis.
"the strong family-level expectation that aldolase participates in cytosolic glycolysis"