2026-10-25: ROOT authorized protected bootstrap after the finite ownership preflight. Exported committed runtime and fixed-main normal bytes into a private workspace, qualified one genuine archived EZH2 publication cache, then ran genuine fetch and caching commands. All 62 seeded contexts remain PENDING. Provider failure remains explicit; no fabricated output was substituted.
Exact raw/TSV/source normalization, literal product reconstruction and source-provenance partition were checked mechanically. Official PANTHER requests returned HTTP 500, so actual target phylogenetic placement remains a task for later evidence review. No science conclusions were drawn from that service failure. The normal family membership and source IBD assertions are preserved separately.
The sole context collapse differs only in ID/date/assigner. No extension, source qualifier, reference identity, natural product or quotation was manually added or deleted. All 232 preimages and eight canonical baseline files remain exact. ROOT retains acceptance, scientific lane assignment and all canonical/history/publication authority.
Reviewed the fifteen database-derived source contexts independently of the direct literature lane: eight ACCEPT, five MODIFY, one KEEP_AS_NON_CORE and one UNDECIDED. The source guide gives the actual source hierarchy, public original locators, species/preparation/product boundaries and access limits.
The broader serine/threonine activity retains additional measured chemistry. The distinct main Figure 5F and supplemental Figure S6F IGFBP1 spectra in PMID:26091039 have different precursor masses and site assignments; the supplementary ambiguity is preserved without collapsing the two results. Calcium binding remains a positive, bounded mouse-derived capacity and is not called calcium-dependent kinase activity.
Golgi/ER membrane refinements use independent human topology rather than pretending coarse mouse or HPA imaging resolved membranes. Reactome's specific processed 93–584 ER-lumen entity remains UNDECIDED with a SUSPECTED/CHECK source query. Real ER residence and kinase activity remain positive; no source record was altered or externally reported.
All sixty-two imported source objects, the forty-seven unassigned PENDING reviews, seventeen inherited reference identities/findings, both literal natural products, description and INITIALIZED status are preserved. Four genuinely fetched donor references are proposed additively. No core functions, NEW annotations or publication quotations were added. The failed provider output was not manufactured. Normal-cache flags and external original access/read scopes are documented separately.
The assigned 47 source contexts are reviewed as 19 ACCEPT, 6 MODIFY, 21 KEEP_AS_NON_CORE and 1 UNDECIDED. The other 15 database contexts remain exactly PENDING at this stage; this is not a whole-gene completion claim.
FAM20C directly transfers phosphate to protein substrates. Serine is strongly preferred, but the actual human IGFBP1 main Figure 5F in PMID:26091039 supports Thr193 as well as Ser194. Its different supplemental S6F peptide has an ambiguous site. The broad kinase and protein-phosphorylation refinements preserve this measured breadth. S1P performs FAM20C proteolysis; Ero1α/PDI perform their own redox/chaperone functions; FAM20A activates the kinase. None of those partner activities is transferred to FAM20C.
Supported generic substrate and transport associations remain non-core under the standing campaign exception. The exact SERPINC1 partner context in the inaccessible 2012 supplemental table remains UNDECIDED, without a wrong-gene allegation or removal from an abstract/main-text omission. The urinary and parotid target-table entries are positive fraction observations, not evidence of an exosome mechanism or demonstrated contamination.
Human precursor membrane topology, stress-dependent WT ER redistribution, secreted Asp93 protein, engineered retention/deletion constructs and the two natural products are kept separate. The natural 252-residue product is not equated with a mature processed chain or recombinant kinase fragment. Current term definitions and typed closures support the precise replacements; no NEW assertion, quotation, core or top-level biological description is authored in this lane.
The source guide gives public paper and experiment locators. Cache availability, external recovery and actual reading are recorded separately in all nine manual reference assessments. No normal cache was changed or added. Failed acquisition responses remain preserved, and the provider failure is not replaced by an invented report. The earlier intake journal is historical; its original target-ancestry access gap is outside this direct lane and does not contradict a separately documented database follow-up.
The two ROOT-accepted source lanes now cover all 62 imported contexts: 27 ACCEPT,
11 MODIFY, 22 KEEP_AS_NON_CORE and two UNDECIDED. Sixty-one complete reviews are
literal copies of the lanes. Row 51 has the explicitly accepted integration refinement
to peptidyl-serine and peptidyl-threonine phosphorylation; its source and MODIFY action
remain unchanged. The 21 references retain 17 original identities/findings plus four
genuine donor additions. Five shared assessments preserve both full lane scopes.
One core represents the performed protein serine/threonine kinase chemistry, its
specific phosphorylation processes and enamel participation. The standalone description
and paired questions/experiments distinguish membrane precursor, secreted Asp93 mature
form and the natural 252-residue product. No physiological metal occupancy, soluble
mature ER pool, universal substrate specificity or natural-product equivalence is claimed.
All source objects, literal products, reference findings and zero quotation anchors
remain preserved. No NEW annotation is added; computed canonical status remains ROOT's
later responsibility, and this private proposal retains INITIALIZED.
The source guide records primary public locators, source-access
and product limits, the main Figure 5F versus supplemental S6F distinction, and the
source-specific ER-lumen question. Normal-cache metadata and all prior source bytes are
unchanged. Provider failure remains explicit; no provider file is manufactured.
FAM20C's matrix phosphorylation contributes to bone/dentin as well as enamel biology.
PMID:22582013, Results/Figure 3
shows WT-versus-D478A phosphorylation of OPN, DMP1 and MEPE; the independent human
enamel-substrate experiments are PMID:25789606, Figure 1 and Methods.
The core now represents biomineral tissue development once, covering that breadth.
The enamel-specific 25789606 review is refined to enamel mineralization, a current
is_a descendant, without retaining redundant parent/child core terms. This supersedes
the enamel-only scope of the preceding core proposal, whose journal text is preserved.
Serine/threonine breadth is supported by the actual 2015 main Results and Figure 5E/F
and the curated threonyl-protein reaction in UniProt Q8IXL6.
The main text names Thr193 alongside Ser45/Ser199. Main Figure 5F's Tp+Sp assignment
and the different, ambiguous S6F peptide remain separately documented with direct
public image/supplement links in the source guide.
No spectral reanalysis is claimed. The same catalytic core explicitly names the
ERO1A/PDI phosphorylation work in PMID:29858230(https://doi.org/10.15252/embj.201798699)
and PMID:32149426(https://doi.org/10.15252/embj.2019103841), without transferring
substrate oxidoreductase/chaperone functions to the kinase.
The SERPINC1 curated pair is acknowledged, but its exact inaccessible 2012 assay
remains UNDECIDED; positive mouse calcium binding remains non-core and is not called
calcium-dependent kinase activity. Shared product caveats are consolidated, with the
two natural products, precursor topology and processed Asp93 chain still distinct.
All 62 imported source objects, 21 prior reference identities/findings, original
access flags and zero quotation anchors remain exact. One UniProt reference is added;
only the enamel-specific review action changes. Prior citation read scopes remain
explicit; the 22582013 page span is corrected to Science 336:1150–1153 from the
inspected official record. The journal is append-only, and no new annotation or
publication quotation is added. The campaign instruction and public locators in the
source guide govern quotation-free evidence documentation.