Gene Ontology annotation through association of InterPro records with GO terms

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

PMID:15659660

Disparate subcellular localization patterns of Pseudomonas aeruginosa Type IV pilus ATPases involved in twitching motility.

Functional YFP-PilT is cytoplasmic and localises to both poles.

"PilB and PilT fusions localized to both poles"

PMID:18174131

Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU.

Purified PilT hydrolyses ATP; Asp Box and His Box residues are required for activity and Walker A/B residues for twitching.

"Assay of purified 6xHis-tagged PilB, PilT and PilU from P. aeruginosa showed that all three proteins have ATPase activities in vitro."

PMID:20595000

P. aeruginosa PilT structures with and without nucleotide reveal a dynamic type IV pilus retraction motor.

PilT is a hexameric retraction motor; structures with and without AMP-PCP.

"Retraction is accomplished by the motor protein PilT."

file:PSEAE/pilT/pilT-uniprot.txt

UniProt entry P24559 (PILT_PSEAE)