GO_REF:0000024

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

Xenopus Dnaaf5 localization to DynAPs supports the human compartment transfer.

GO_REF:0000033

Annotation inferences using phylogenetic trees

Five source assertions trace to the human DNAAF5 family lineage and experimental human, fly and Xenopus grounding.

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

UniProt location SL-0542 maps to dynein axonemal particle.

GO_REF:0000052

Gene Ontology annotation based on curation of immunofluorescence data

Human ICC/IF supports a cytosolic DNAAF5 pool in U2OS and ASC52telo cells.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

The current ARBA rule assigns the broad cytoplasmic compartment through a matching DNAAF5 FunFam.

PMID:23040496

Whole-exome capture and sequencing identifies HEATR2 mutation as a cause of primary ciliary dyskinesia.

Human HEATR2 deficiency and ortholog silencing disrupt dynein arms and ciliary motility, with cytoplasmic rather than ciliary localization.

PMID:25232951

HEATR2 plays a conserved role in assembly of the ciliary motile apparatus.

Human and fly HEATR2 loss disrupt outer and inner dynein-arm assembly; endogenous human HEATR2 associates with DNAI2.

PMID:29358401

Establishment of the early cilia preassembly protein complex during motile ciliogenesis.

Human HEATR2 associates with SPAG1 and itself; DNAAF2 association is compatible with an indirect SPAG1-mediated early preassembly complex.

PMID:30561330

A liquid-like organelle at the root of motile ciliopathy.

Xenopus Heatr2 localizes to dynein-containing cytoplasmic particles; its depletion reduces particle numbers and alters Ktu mobility.

PMID:33263282

Functional partitioning of a liquid-like organelle during assembly of axonemal dyneins.

Xenopus Dnaaf5 marks DynAPs containing chaperones and spatially partitioned outer/inner dynein subunits.

UniProtKB:Q86Y56

Dynein axonemal assembly factor 5 (DNAAF5), Homo sapiens

The reviewed sequence record defines three natural products of 855, 843 and 235 residues, including an alternative C terminus and a shorter C-terminal product.