Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Cloning and sequence analysis of a trans-regulatory locus required for exoenzyme S synthesis in Pseudomonas aeruginosa.
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ExsA, an AraC-family homolog, is required for induction of exoenzyme S synthesis above a basal level.
"The ExsA gene product appeared to be required for induction of exoenzyme S synthesis above a low basal level."
Characterization of ExsA and of ExsA-dependent promoters required for expression of the Pseudomonas aeruginosa type III secretion system.
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ExsA binds T3SS promoters with high affinity; two monomers bind adjacent sites.
"ExsA(His) specifically bound T3SS promoters with high affinity as determined by electrophoretic mobility shift assays (EMSA)."
Functional domains of ExsA, the transcriptional activator of the Pseudomonas aeruginosa type III secretion system.
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The ExsA C-terminal domain binds promoters and activates transcription.
"we show that the CTD of ExsA binds to ExsA-dependent promoters in vitro and activates transcription from ExsA-dependent promoters both in vitro and in vivo"
ExsD inhibits expression of the Pseudomonas aeruginosa type III secretion system by disrupting ExsA self-association and DNA binding activity.
The distal ExsA-binding site in Pseudomonas aeruginosa type III secretion system promoters is the primary determinant for promoter-specific properties.
ExsA and LcrF recognize similar consensus binding sites, but differences in their oligomeric state influence interactions with promoter DNA.
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ExsA consensus-site mutations prevent activation of T3SS promoters.
"Mutations that disrupt the ExsA consensus binding sites in both P. aeruginosa and Y. pestis T3SS promoters prevent activation by ExsA and LcrF."
Analyses of the DNA-binding and transcriptional activation properties of ExsA, the transcriptional activator of the Pseudomonas aeruginosa exoenzyme S regulon.
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Purified ExsA binds ExsA-dependent promoters; the consensus is required for activation.
"ExsA was produced and purified as a fusion protein, MALA3A2, which was shown to bind specifically to promoter regions"