Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry for PSEPK IlvA-I
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Assigns Q88HB4 to EC 4.3.1.19, the biosynthetic threonine-deaminase family, and 2-oxobutanoate production for isoleucine biosynthesis.
"Full=L-threonine dehydratase"
QuickGO annotation snapshot for PSEPK IlvA-I
OpenScientist functional report for PSEPK IlvA-I
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The report explicitly lacks direct Q88HB4 experimental characterization and separately compares the second KT2440 paralog.
"No single experimental study characterizes Q88HB4 directly"
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Physiological relevance of the serine side reaction in KT2440 is untested.
"**Physiological significance of the serine-dehydratase side reaction / 2AA output in KT2440 is untested.**"
Biochemical Characterization of the Mycobacterium smegmatis Threonine Deaminase.
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Direct M. smegmatis threonine-deaminase assays show secondary L-serine acceptance; this supports family activity rather than target-specific kinetics.
"We show here that in addition to l-threonine, l-allo-threonine and l-serine are
also used as substrates by TD"
Analyses of variants of the Ser/Thr dehydratase IlvA provide insight into 2-aminoacrylate metabolism in Salmonella enterica.
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Salmonella experiments distinguish serine-derived 2-aminoacrylate from threonine-derived 2-aminocrotonate and connect enzyme activity to physiological effects.
"the enzyme generates both 2AA (from serine) and 2AC (from threonine)"